| Product Number |
ARP40630_T100 |
| Product Page |
www.avivasysbio.com/pcbp1-antibody-middle-region-arp40630-t100.html |
| Name |
PCBP1 Antibody - middle region (ARP40630_T100) |
| Protein Size (# AA) |
356 amino acids |
| Molecular Weight |
39kDa |
| NCBI Gene Id |
5093 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Poly(rC) binding protein 1 |
| Alias Symbols |
HNRPX, HNRPE1, hnRNP-X, HEL-S-85, hnRNP-E1 |
| Peptide Sequence |
Synthetic peptide located within the following region: CSDAVGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAM |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Zhong,N., (2005) Biochem. Biophys. Res. Commun. 338 (2), 855-861 |
| Description of Target |
PCBP1 appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP1 is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.This intronless gene is thought to be generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding proteins. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. |
| Protein Interactions |
HUWE1; UBC; SNRPA; TP53; SUMO2; SUMO3; TUBGCP3; STAU1; ERG; EED; TUBB6; TUBB2A; PFKP; PFKM; SRPK2; SRPK1; SRPK3; FBXO6; TARDBP; UBD; ADRB2; NPM1; LMNA; HMGA1; CD81; PAN2; PRPF8; PPIG; PRPF3; UBL4A; ANP32A; VCAM1; NOS2; ITGA4; FN1; ATF2; ZNF830; ZCCHC10; P |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PCBP1 (ARP40630_T100) antibody |
| Blocking Peptide |
For anti-PCBP1 (ARP40630_T100) antibody is Catalog # AAP40630 (Previous Catalog # AAPP10617) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human PCBP1 |
| Uniprot ID |
Q15365 |
| Protein Name |
Poly(rC)-binding protein 1 |
| Sample Type Confirmation |
PCBP1 is supported by BioGPS gene expression data to be expressed in HepG2 |
| Protein Accession # |
NP_006187 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_006196 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
PCBP1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 92%; Guinea Pig: 93%; Horse: 93%; Human: 93%; Mouse: 93%; Rabbit: 93%; Rat: 93% |
| Image 1 | Mouse Brain
 | Host: Mouse
Target Name: PCBP1
Sample Tissue: Mouse Brain
Antibody Dilution: 1ug/ml |
|
| Image 2 | Human HepG2
 | WB Suggested Anti-PCBP1 Antibody Titration: 1.25ug/ml
Positive Control: HepG2 cell lysatePCBP1 is supported by BioGPS gene expression data to be expressed in HepG2 |
|
| Image 3 | HepG2
 | Host: Rabbit
Target Name: PCBP1
Sample Type: HepG2
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 2.5ug/mL
Peptide Concentration: 2.0ug/mL
Lysate Quantity: 25ug/lane
Gel Concentration: 12%PCBP1 is supported by BioGPS gene expression data to be expressed in HepG2 |
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