SF3B4 Antibody - N-terminal region (ARP40610_P050)

• Product Number: ARP40610_P050
• Name: SF3B4 Antibody - N-terminal region (ARP40610_P050)
• Protein Size (# AA): 424 amino acids
• Molecular Weight: 47kDa
• Subunit: 4
• NCBI Gene Id: 10262
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Splicing factor 3b, subunit 4, 49kDa
• Alias Symbols: AFD1, Hsh49, SAP49, SF3b49
• Peptide Sequence: Synthetic peptide located within the following region: SFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Nishanian,T.G. (2004) Biochem. Biophys. Res. Commun. 323 (1), 91-97
• Description of Target: SF3B4 is one of four subunits of the splicing factor 3B. The protein cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.
• Protein Interactions: NCK2; METTL17; ZCCHC10; UBASH3A; NUB1; HSPB7; PPP1R16B; SS18L1; HNRNPUL1; SF3B2; BAIAP2; SEC24D; FAM115A; RBM39; BAG3; CWF19L2; MAPK9; FUS; FRK; FKBP1A; STAU1; UBC; SUMO1; SUZ12; RNF2; PRPF40A; WBP4; APBB1; SRPK3; DDX42; PRPF8; CHERP; DDX17; BCAS2; EFTUD2
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-SF3B4 (ARP40610_P050) antibody is Catalog # AAP40610 (Previous Catalog # AAPP22774)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SF3B4
• Uniprot ID: Q15427
• Protein Name: Splicing factor 3B subunit 4
• Sample Type Confirmation:

  SF3B4 is supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_005841
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005850
• Tested Species Reactivity: Human
• Gene Symbol: SF3B4
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 79%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 87%; Zebrafish: 100%
• Image 1: Human Jurkat
  
  WB Suggested Anti-SF3B4 Antibody Titration: 0.2-1 ug/ml
  Positive Control: Jurkat cell lysateSF3B4 is supported by BioGPS gene expression data to be expressed in Jurkat