NCL Antibody - C-terminal region (ARP40584_T100)

Data Sheet
 
Product Number ARP40584_T100
Product Page www.avivasysbio.com/ncl-antibody-c-terminal-region-arp40584-t100.html
Name NCL Antibody - C-terminal region (ARP40584_T100)
Protein Size (# AA) 710 amino acids
Molecular Weight 78kDa
NCBI Gene Id 4691
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Nucleolin
Alias Symbols C23, Nsr1
Peptide Sequence Synthetic peptide located within the following region: GGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Huang,Y., (2006) Blood 107 (9), 3564-3571
Description of Target Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Protein Interactions HUWE1; PIK3R1; PA2G4; NCL; HNRNPD; AURKA; MDM2; NFE4; CDKN1A; ADAP1; STAU1; ADAP2; UBC; NUP62; ZBTB1; ARFGEF1; RPA3; RPA2; RPA1; ERG; RNF2; SUZ12; EED; EZH2; BMI1; NPM3; EIF3G; XRCC5; PLOD2; NCBP1; MCM2; LMNA; PRMT1; HNRNPK; KCTD12; DNM1; ACLY; SRPK3; TAR
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-NCL (ARP40584_T100) antibody
Blocking Peptide For anti-NCL (ARP40584_T100) antibody is Catalog # AAP40584 (Previous Catalog # AAPP22760)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human NCL
Uniprot ID P19338
Protein Name Nucleolin
Sample Type Confirmation

NCL is supported by BioGPS gene expression data to be expressed in Daudi

Protein Accession # NP_005372
Purification Protein A purified
Nucleotide Accession # NM_005381
Tested Species Reactivity Human
Gene Symbol NCL
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%
Image 1
Human Daudi
WB Suggested Anti-NCL Antibody Titration: 1.25ug/ml
Positive Control: Daudi cell lysateNCL is supported by BioGPS gene expression data to be expressed in Daudi
Image 2
Human NT-2
Sample Type:
Human brain stem cells
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution:
1:1000
Color/Signal Descriptions:
NCL: Red DAPI:Blue
Gene Name:
NCL
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science
Image 3
Human Kidney
Rabbit Anti-NCL Antibody
Catalog Number: ARP40584
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
Image 4
Human NT-2 s
Sample Type: 1. Human NT-2 cells (60ug)
Primary Antibody Dilution: 2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution: 1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical Science 
 

AVIVA SYSTEMS BIOLOGY manufactures and sells quality antibody products covering genome wide proteins.

AVIVA SYSTEMS BIOLOGY
10211 Pacific Mesa Blvd, Ste 401, San Diego, CA 92121 USA | Tel: (858)552-6979 | info@avivasysbio.com