Product Number |
ARP40584_T100 |
Product Page |
www.avivasysbio.com/ncl-antibody-c-terminal-region-arp40584-t100.html |
Name |
NCL Antibody - C-terminal region (ARP40584_T100) |
Protein Size (# AA) |
710 amino acids |
Molecular Weight |
78kDa |
NCBI Gene Id |
4691 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Nucleolin |
Alias Symbols |
C23, Nsr1 |
Peptide Sequence |
Synthetic peptide located within the following region: GGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Huang,Y., (2006) Blood 107 (9), 3564-3571 |
Description of Target |
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. |
Protein Interactions |
HUWE1; PIK3R1; PA2G4; NCL; HNRNPD; AURKA; MDM2; NFE4; CDKN1A; ADAP1; STAU1; ADAP2; UBC; NUP62; ZBTB1; ARFGEF1; RPA3; RPA2; RPA1; ERG; RNF2; SUZ12; EED; EZH2; BMI1; NPM3; EIF3G; XRCC5; PLOD2; NCBP1; MCM2; LMNA; PRMT1; HNRNPK; KCTD12; DNM1; ACLY; SRPK3; TAR |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-NCL (ARP40584_T100) antibody |
Blocking Peptide |
For anti-NCL (ARP40584_T100) antibody is Catalog # AAP40584 (Previous Catalog # AAPP22760) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human NCL |
Uniprot ID |
P19338 |
Protein Name |
Nucleolin |
Sample Type Confirmation |
NCL is supported by BioGPS gene expression data to be expressed in Daudi |
Protein Accession # |
NP_005372 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_005381 |
Tested Species Reactivity |
Human |
Gene Symbol |
NCL |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100% |
Image 1 | Human Daudi
| WB Suggested Anti-NCL Antibody Titration: 1.25ug/ml Positive Control: Daudi cell lysateNCL is supported by BioGPS gene expression data to be expressed in Daudi |
|
Image 2 | Human NT-2
| Sample Type: Human brain stem cellsPrimary Antibody Dilution: 1:500Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution: 1:1000Color/Signal Descriptions: NCL: Red DAPI:BlueGene Name: NCLSubmitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science |
|
Image 3 | Human Kidney
| Rabbit Anti-NCL Antibody Catalog Number: ARP40584 Paraffin Embedded Tissue: Human Kidney Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X |
|
Image 4 | Human NT-2 s
| Sample Type: 1. Human NT-2 cells (60ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical Science |
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