SFPQ Antibody - N-terminal region (ARP40572_T100)

• Product Number: ARP40572_T100
• Name: SFPQ Antibody - N-terminal region (ARP40572_T100)
• Protein Size (# AA): 707 amino acids
• Molecular Weight: 78kDa
• NCBI Gene Id: 6421
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Splicing factor proline/glutamine-rich
• Alias Symbols: PSF, POMP100, PPP1R140
• Peptide Sequence: Synthetic peptide located within the following region: VPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGE
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Rosonina,E., (2005) Mol. Cell. Biol. 25 (15), 6734-6746
• Description of Target: SFPQ is DNA- and RNA binding protein. It is essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. It binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. It interacts with U5 snRNA. It may be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. SFPQ may be involved in homologous DNA pairing. SFPQ is involved in transcriptional regulation. It binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
• Protein Interactions: SNRPA; PTBP1; NONO; HDAC2; FUS; HUWE1; UBC; SUMO2; MDM2; STAU1; RPA3; RPA2; RPA1; ERG; ASB18; ASB12; WWOX; ASB3; EED; rev; TIMM23B; APBB1; PIN1; WBP4; TCERG1; GAS7; PRPF40A; PARK2; UBD; CD81; PAN2; RNF43; WHSC1; VCAM1; SMAD5; ITGA4; FN1; CSNK2A1; BTK; DNA
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-SFPQ (ARP40572_T100) antibody is Catalog # AAP40572 (Previous Catalog # AAPP22332)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SFPQ
• Uniprot ID: P23246
• Protein Name: Splicing factor, proline- and glutamine-rich
• Sample Type Confirmation:

  SFPQ is strongly supported by BioGPS gene expression data to be expressed in HeLa, HepG2

• Protein Accession #: NP_005057
• Purification: Protein A purified
• Nucleotide Accession #: NM_005066
• Tested Species Reactivity: Human
• Gene Symbol: SFPQ
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Human: 100%; Mouse: 79%; Rat: 79%
• Image 1: Human HeLa
  
  Lanes:
  Lane 1: 50ug Hela Lysate
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Anti-rabbit-HRP
  Secondary Antibody Dilution:
  1:10,000
  Gene Name:
  SFPQ
  Submitted by:
  Archa Fox, University of Western Australia
  SFPQ is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells
• Image 2: Human Heart
  
  Rabbit Anti-SFPQ antibody
  Catalog Number: ARP40572
  Paraffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 3: Human HepG2
  
  WB Suggested Anti-SFPQ Antibody Titration: 1.25ug/ml
  ELISA Titer: 1:12500
  Positive Control: HepG2 cell lysate.SFPQ is strongly supported by BioGPS gene expression data to be expressed in HepG2
• Image 4: Human Skin
  
  Rabbit Anti-SFPQ Antibody
  Catalog Number: ARP40572
  Paraffin Embedded Tissue: Human Skin
  Cellular Data: Squamous epithelial cells
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 5: Human Intestine
  
  Rabbit Anti-SFPQ Antibody
  Catalog Number: ARP40572
  Paraffin Embedded Tissue: Human Intestine
  Cellular Data: Epithelial cells of intestinal villas
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X