HNRPF Antibody - C-terminal region (ARP40565_T100)

Data Sheet
 
Product Number ARP40565_T100
Product Page www.avivasysbio.com/hnrpf-antibody-c-terminal-region-arp40565-t100.html
Name HNRPF Antibody - C-terminal region (ARP40565_T100)
Protein Size (# AA) 415 amino acids
Molecular Weight 46kDa
NCBI Gene Id 3185
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Heterogeneous nuclear ribonucleoprotein F
Alias Symbols HNRPF, mcs94-1, OK/SW-cl.23
Peptide Sequence Synthetic peptide located within the following region: LNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNS
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Balasubramani,M., (2006) Cancer Res. 66 (2), 763-769
Description of Target HNRPF belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH.
Protein Interactions HSFY1; C1orf94; CCDC33; EIF4ENIF1; RBFOX2; IKZF3; HNRNPUL1; HNRNPM; FUS; TFG; DZIP3; UBC; AES; NEDD1; CEP250; SUMO2; SUMO3; TP53; CDC20; STAU1; IVNS1ABP; SUMO1; NEDD8; MDM2; WWOX; RPA3; RPA2; RPA1; ERG; EED; rev; PRPF40A; OSGEP; ABCF3; DNAAF5; PPME1; PPP1
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Enhanced Validation
Relative Expression (Western Blot) Avivasheild
Datasheets/Manuals Printable datasheet for anti-HNRNPF (ARP40565_T100) antibody
Blocking Peptide For anti-HNRNPF (ARP40565_T100) antibody is Catalog # AAP40565 (Previous Catalog # AAPP22743)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human HNRPF
Uniprot ID P52597
Protein Name Heterogeneous nuclear ribonucleoprotein F
Publications

White, R. et al. Heterogeneous nuclear ribonucleoprotein (hnRNP) F is a novel component of oligodendroglial RNA transport granules contributing to regulation of myelin basic protein (MBP) synthesis. J. Biol. Chem. 287, 1742-54 (2012). 22128153

Sample Type Confirmation

HNRNPF is supported by BioGPS gene expression data to be expressed in HepG2

Protein Accession # NP_004957
Purification Protein A purified
Nucleotide Accession # NM_004966
Tested Species Reactivity Human
Gene Symbol HNRNPF
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 91%; Dog: 92%; Guinea Pig: 100%; Horse: 92%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Image 1
Human Lung
Rabbit Anti-HNRNPF Antibody
Catalog Number: ARP40565
Paraffin Embedded Tissue: Human alveolar cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
Image 2
Human Kidney
Rabbit Anti-HNRPF Antibody
Catalog Number: ARP40565
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
Image 3

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Protein is modified by phosphorylation.
 

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