Product Number |
ARP40565_T100 |
Product Page |
www.avivasysbio.com/hnrpf-antibody-c-terminal-region-arp40565-t100.html |
Name |
HNRPF Antibody - C-terminal region (ARP40565_T100) |
Protein Size (# AA) |
415 amino acids |
Molecular Weight |
46kDa |
NCBI Gene Id |
3185 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Heterogeneous nuclear ribonucleoprotein F |
Alias Symbols |
HNRPF, mcs94-1, OK/SW-cl.23 |
Peptide Sequence |
Synthetic peptide located within the following region: LNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNS |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Balasubramani,M., (2006) Cancer Res. 66 (2), 763-769 |
Description of Target |
HNRPF belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. |
Protein Interactions |
HSFY1; C1orf94; CCDC33; EIF4ENIF1; RBFOX2; IKZF3; HNRNPUL1; HNRNPM; FUS; TFG; DZIP3; UBC; AES; NEDD1; CEP250; SUMO2; SUMO3; TP53; CDC20; STAU1; IVNS1ABP; SUMO1; NEDD8; MDM2; WWOX; RPA3; RPA2; RPA1; ERG; EED; rev; PRPF40A; OSGEP; ABCF3; DNAAF5; PPME1; PPP1 |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Enhanced Validation |
Relative Expression (Western Blot) |
|
|
Datasheets/Manuals |
Printable datasheet for anti-HNRNPF (ARP40565_T100) antibody |
Blocking Peptide |
For anti-HNRNPF (ARP40565_T100) antibody is Catalog # AAP40565 (Previous Catalog # AAPP22743) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human HNRPF |
Uniprot ID |
P52597 |
Protein Name |
Heterogeneous nuclear ribonucleoprotein F |
Publications |
White, R. et al. Heterogeneous nuclear ribonucleoprotein (hnRNP) F is a novel component of oligodendroglial RNA transport granules contributing to regulation of myelin basic protein (MBP) synthesis. J. Biol. Chem. 287, 1742-54 (2012). 22128153 |
Sample Type Confirmation |
HNRNPF is supported by BioGPS gene expression data to be expressed in HepG2 |
Protein Accession # |
NP_004957 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_004966 |
Tested Species Reactivity |
Human |
Gene Symbol |
HNRNPF |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 91%; Dog: 92%; Guinea Pig: 100%; Horse: 92%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100% |
Image 1 | Human Lung
| Rabbit Anti-HNRNPF Antibody Catalog Number: ARP40565 Paraffin Embedded Tissue: Human alveolar cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X |
|
Image 2 | Human Kidney
| Rabbit Anti-HNRPF Antibody Catalog Number: ARP40565 Paraffin Embedded Tissue: Human Kidney Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X |
|
Image 3 |
| 25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Protein is modified by phosphorylation. |
|