SSB Antibody - N-terminal region (ARP40462_P050)

• Product Number: ARP40462_P050
• Name: SSB Antibody - N-terminal region (ARP40462_P050)
• Protein Size (# AA): 408 amino acids
• Molecular Weight: 45kDa
• NCBI Gene Id: 6741
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Sjogren syndrome antigen B (autoantigen La)
• Alias Symbols: La, LARP3, La/SSB
• Peptide Sequence: Synthetic peptide located within the following region: ISEDKTKIRRSPSKPLPEVTDEYKNDVKNRSVYIKGFPTDATLDDIKEWL
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Teplova,M., (2006) Mol. Cell 21 (1), 75-85
• Description of Target: SSB is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(OH) elements of newly RNA polymerase III-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. SSB protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome and systemic lupus erythematosus.La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(OH) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome (MIM 270150) and systemic lupus erythematosus (SLE; MIM 152700) (Teplova et al., 2006).[supplied by OMIM].
• Protein Interactions: HUWE1; UBC; MDM2; LIN28A; BMI1; SUZ12; RNF2; rev; NPLOC4; DNM1L; UBA2; ANP32A; VBP1; RABGGTA; PPP2CA; KARS; CTTN; TARDBP; SOX2; HMGA1; ARFGEF1; NCL; GZMH; CSNK2B; CSNK2A1; HCVgp1; CBX6; SPIB; IL7R; IFIT3; FN1; SMURF1; BARD1; PAXIP1; APP; TROVE2; XIAP; CAN
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: IHC Information: Human Prostate (formalin-fixed, paraffin-embedded) stained with SSB antibody ARP40462_P050 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
• Blocking Peptide: For anti-SSB (ARP40462_P050) antibody is Catalog # AAP40462 (Previous Catalog # AAPP22230)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SSB
• Uniprot ID: P05455
• Protein Name: Lupus La protein
• Sample Type Confirmation:

  SSB is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_003133
• Purification: Affinity Purified
• Nucleotide Accession #: NM_003142
• Tested Species Reactivity: Human
• Gene Symbol: SSB
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human Jurkat
  
  WB Suggested Anti-SSB Antibody Titration: 0.25 ug/mlPositive Control: Jurkat cell lysateSSB is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
• Image 2: Jurkat
  
  Host: Rabbit
  Target Name: SSB
  Sample Type: Jurkat
  Lane A: Primary Antibody
  Lane B: Primary Antibody + Blocking Peptide
  Primary Antibody Concentration: 2.0ug/mL
  Peptide Concentration: 2.0ug/mL
  Lysate Quantity: 25ug/lane
  Gel Concentration: 12%
• Image 3: Human Heart
  
  Rabbit Anti-SSB Antibody
  Catalog Number: ARP40462
  Paraffin Embedded Tissue: Human cardiac cell
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 4: Human Kidney
  
  Rabbit Anti-SSB Antibody
  Catalog Number: ARP40462
  Paraffin Embedded Tissue: Human Kidney
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 5: Human Liver
  
  nti-SSB antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
• Image 6: Human Prostate
  
  Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-SSB antibody (ARP40462_P050)