| Product Number |
ARP40368_P050 |
| Product Page |
www.avivasysbio.com/hnrpl-antibody-n-terminal-region-arp40368-p050.html |
| Name |
HNRPL Antibody - N-terminal region (ARP40368_P050) |
| Protein Size (# AA) |
589 amino acids |
| Molecular Weight |
65kDa |
| NCBI Gene Id |
3191 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Heterogeneous nuclear ribonucleoprotein L |
| Description |
|
| Alias Symbols |
HNRPL, hnRNP-L, P/OKcl.14 |
| Peptide Sequence |
Synthetic peptide located within the following region: AAGGGGGGENYDDPHKTPASPVVHIRGLIDGVVEADLVEALQEFGPISYV |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Guang,S., (2005) Mol. Cell. Biol. 25 (15), 6303-6313 |
| Description of Target |
Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions.Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. |
| Protein Interactions |
TP53; FUS; TRIM68; SUMO2; SUMO3; IVNS1ABP; UBC; MDM2; RPA3; RPA2; RPA1; ILF3; HNRNPA2B1; EED; VHL; VEGFA; RNF2; NEDD4; ITCH; FBXO6; HMGA1; vif; TP63; UBL4A; WHSC1; VCAM1; ITGA4; IL7R; IFIT3; IFIT2; FN1; DAB2; CSNK2A1; EIF4A3; MAGOH; HNRNPA3; NPLOC4; LSM14 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-HNRNPL (ARP40368_P050) antibody |
| Blocking Peptide |
For anti-HNRNPL (ARP40368_P050) antibody is Catalog # AAP40368 (Previous Catalog # AAPP22112) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human HNRPL |
| Uniprot ID |
P14866 |
| Protein Name |
Heterogeneous nuclear ribonucleoprotein L |
| Publications |
MyoD induced enhancer RNA interacts with hnRNPL to activate target gene transcription during myogenic differentiation. Nat Commun. 10, 5787 (2019). 31857580
Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription. Cell. 178, 107-121.e18 (2019). 31251911 |
| Sample Type Confirmation |
HNRNPL is strongly supported by BioGPS gene expression data to be expressed in Jurkat |
| Protein Accession # |
NP_001524 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001533 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
HNRNPL |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
| Application |
IF, IP, WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100% |
| Image 1 | Mouse Liver
 | Host: Mouse
Target Name: HNRNPL
Sample Tissue: Mouse Liver
Antibody Dilution: 1ug/ml |
|
| Image 2 | Human Liver
 | Rabbit Anti-HNRPL Antibody
Catalog Number: ARP40368
Paraffin Embedded Tissue: Human Liver
Cellular Data: Hepatocytes
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 3 | Human HepG2 Whole Cell
 | Host: Rabbit
Target Name: HNRNPL
Sample Tissue: Human HepG2 Whole Cell
Antibody Dilution: 1ug/ml |
|
| Image 4 | Human Jurkat
 | WB Suggested Anti-HNRPL Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysateHNRNPL is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells |
|
| Image 5 | HeLa S3, MCF7, K562
 | Lane 1: 20ug HeLa S3 lysate Lane 2: 20ug MCF7 lysate Lane 3: 20ug K562 lysate
Primary Antibody Dilution:
1:4000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
HNRPL
Submitted by:
Anonymous |
|
| Image 6 | MCF-7
 | Sample Type:
MCF7 cells
Primary Antibody Dilution:
1:200
Secondary Antibody:
Anti-rabbit-FITC
Secondary Antibody Dilution:
1:500
Color/Signal Descriptions:
DAPI: Blue hnRNPL: Green
Gene Name:
HNRPL
Submitted by:
Anonymous |
|
| Image 7 | K562
 | Amount and Sample Type:
Lane 1: 5% InputLane 2: 5% SupLane 3: Normal IgGLane 4: hn-RNPL ppt. k562 sample
IP Antibody:
HNRPL
Amount of IP Antibody:
Primary Antibody:
HNRPL
Primary Antibody Dilution:
1:4000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
HNRPL
Submitted by:
Anonymous |
|
| Image 8 | Human Heart
 | Rabbit Anti-HNRPL antibody
Catalog Number: ARP40368
Paraffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
