U2AF1 Antibody - N-terminal region (ARP40326_P050)

Data Sheet
 
Product Number ARP40326_P050
Product Page www.avivasysbio.com/u2af1-antibody-n-terminal-region-arp40326-p050.html
Name U2AF1 Antibody - N-terminal region (ARP40326_P050)
Protein Size (# AA) 240 amino acids
Molecular Weight 26 kDa
NCBI Gene Id 7307
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name U2 small nuclear RNA auxiliary factor 1
Alias Symbols RN, FP793, U2AF35, U2AFBP, RNU2AF1
Peptide Sequence Synthetic peptide located within the following region: MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTILIQN
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Rino,J., (2008) Mol. Cell. Biol. 28 (9), 3045-3057
Description of Target This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which pl
Protein Interactions SRPK2; HNRNPK; CLK2; AP2B1; U2AF2; RNPS1; NDN; SUMO3; UBC; SUZ12; TERF1; RNF2; EZH2; rev; JMJD6; SUV39H1; SRPK1; SRPK3; CIR1; SF3B2; CHERP; DHX38; PRPF4; RBM10; VEGFA; SNRPD1; SRSF2; SRSF1; MFAP1; IL7R; FN1; FRA10AC1; TOE1; PRPF38A; NKAP; PRPF40A; ZCCHC10
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Enhanced Validation
Relative Expression (Western Blot) Avivasheild
Datasheets/Manuals Printable datasheet for anti-U2AF1 (ARP40326_P050) antibody
Blocking Peptide For anti-U2AF1 (ARP40326_P050) antibody is Catalog # AAP40326 (Previous Catalog # AAPP10363)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human U2AF1
Uniprot ID Q701P4
Protein Name Splicing factor U2AF 35 kDa subunit
Protein Accession # NP_001020374
Purification Affinity Purified
Nucleotide Accession # NM_001025203
Tested Species Reactivity Human
Gene Symbol U2AF1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Goat: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 86%; Zebrafish: 100%
Image 1
Human Fetal stomach
WB Suggested Anti-U2AF1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positive Control: Fetal stomach
Image 2
Western Blot
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Protein migrates aberrantly at ~32 kDa.
 

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