U2AF2 Antibody - N-terminal region (ARP40284_T100)

• Product Number: ARP40284_T100
• Name: U2AF2 Antibody - N-terminal region (ARP40284_T100)
• Protein Size (# AA): 471 amino acids
• Molecular Weight: 52kDa
• NCBI Gene Id: 11338
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: U2 small nuclear RNA auxiliary factor 2
• Alias Symbols: U2AF65
• Peptide Sequence: Synthetic peptide located within the following region: EFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSAS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Sickmier,E.A., Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 62 (PT
• Description of Target: U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 is the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly.U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
• Protein Interactions: THAP1; PUF60; U2AF1; SNRPA; AES; DACH1; SUMO3; UBC; SUZ12; EED; TERF1; EZH2; RNF2; PRPF40A; TCERG1; APBB1; PIN1; SH3RF2; SRPK2; SRPK1; SF1; SRSF1; RBM39; QKI; SCAF11; PRPF3; RBM10; RBM4; SMAD5; IL7R; HNRNPK; HNRNPD; HLA-DMA; SUGP1; CCAR1; SAP30BP; RBFOX2;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-U2AF2 (ARP40284_T100) antibody is Catalog # AAP40284 (Previous Catalog # AAPP22992)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human U2AF2
• Uniprot ID: Q96HC5
• Protein Name: Splicing factor U2AF 65 kDa subunit
• Sample Type Confirmation:

  U2AF2 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_001012496
• Purification: Protein A purified
• Nucleotide Accession #: NM_001012478
• Tested Species Reactivity: Human
• Gene Symbol: U2AF2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 93%
• Image 1: Human Jurkat
  
  Host: Rabbit
  Target Name: U2AF2
  Sample Tissue: Human Jurkat
  Antibody Dilution: 1.0ug/ml