| Product Number |
ARP40249_T100 |
| Product Page |
www.avivasysbio.com/sf3b1-antibody-n-terminal-region-arp40249-t100.html |
| Name |
SF3B1 Antibody - N-terminal region (ARP40249_T100) |
| Protein Size (# AA) |
1304 amino acids |
| Molecular Weight |
146 kDa |
| Subunit |
1 |
| NCBI Gene Id |
23451 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Splicing factor 3b, subunit 1, 155kDa |
| Alias Symbols |
MDS, PRP10, Hsh155, PRPF10, SAP155, SF3b155 |
| Peptide Sequence |
Synthetic peptide located within the following region: MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSR |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Beausoleil,S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135 |
| Description of Target |
SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Protein Interactions |
SART3; SUMO2; SUMO3; MDM2; STAU1; UBC; LGR4; WWOX; RPA3; RPA2; RPA1; ERG; EED; RNF2; EZH2; SUZ12; APBB1; PRPF40A; WBP4; TCERG1; FBXO6; RBM17; SNIP1; SF3B6; WHSC1; RPS2; RPL23A; RPL18A; RPL10; RPL3; PLRG1; SMAD9; SMAD5; SMAD1; IL7R; FN1; ESR1; CSNK2A1; EIF |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Enhanced Validation |
| Relative Expression (Western Blot) |
 |
|
| Datasheets/Manuals |
Printable datasheet for anti-SF3B1 (ARP40249_T100) antibody |
| Blocking Peptide |
For anti-SF3B1 (ARP40249_T100) antibody is Catalog # AAP40249 (Previous Catalog # AAPS02808) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human SF3B1 |
| Uniprot ID |
O75533 |
| Protein Name |
Splicing factor 3B subunit 1 |
| Protein Accession # |
NP_036565 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_012433 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
SF3B1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86% |
| Image 1 | Human Lung
 | Rabbit Anti-SF3B1 Antibody
Catalog Number: ARP40249
Paraffin Embedded Tissue: Human alveolar cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 2 | Human Liver
 | Rabbit Anti-SF3B1 Antibody
Catalog Number: ARP40249
Paraffin Embedded Tissue: Human Liver
Cellular Data: Hepatocytes
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 3 | Human Skin
 | Rabbit Anti-SF3B1 Antibody
Catalog Number: ARP40249
Paraffin Embedded Tissue: Human Skin
Cellular Data: Epidermal cells
Antibody Concentration: 16 ug/ml
Magnification: 400X |
|
| Image 4 |
 | 25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. The peptide sequence is also in an isoform of 54 kDa and the protein may be modified via citrullination, sumoylation, and/or phosphorylation.
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