HIF3A Antibody - C-terminal region (ARP39936_T100)

• Product Number: ARP39936_T100
• Name: HIF3A Antibody - C-terminal region (ARP39936_T100)
• Protein Size (# AA): 667 amino acids
• Molecular Weight: 72 kDa
• NCBI Gene Id: 64344
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Hypoxia inducible factor 3, alpha subunit
• Alias Symbols: IPAS, MOP7, PASD7, HIF-3A, bHLHe17, HIF3-alpha-1
• Peptide Sequence: Synthetic peptide located within the following region: PGSLQDPSTPLLNLNEPLGLGPSLLSPYSDEDTTQPGGPFQPRAGSAQAD
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Kimura,K., (2006) Genome Res. 16 (1), 55-65
• Description of Target: HIF3A is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression.The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. At least three transcript variants encoding three different isoforms have been found for this gene.
• Protein Interactions: HSP90AA1; VHL; EGLN3; EGLN1; EGLN2; HIF1A;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-HIF3A (ARP39936_T100) antibody is Catalog # AAP39936 (Previous Catalog # AAPP23237)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HIF3A
• Uniprot ID: Q9Y2N7
• Protein Name: Hypoxia-inducible factor 3-alpha
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that HIF3A is expressed in HepG2

• Protein Accession #: NP_690007
• Purification: Protein A purified
• Nucleotide Accession #: NM_152794
• Tested Species Reactivity: Human
• Gene Symbol: HIF3A
• Predicted Species Reactivity: Human
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Image 1: Human HepG2
  
  WB Suggested Anti-HIF3A Antibody Titration: 1.25ug/ml
  Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that HIF3A is expressed in HepG2
• Image 2: Human kidney
  
  Human kidney