TARDBP Antibody - C-terminal region (ARP38942_T100)

• Product Number: ARP38942_T100
• Name: TARDBP Antibody - C-terminal region (ARP38942_T100)
• Protein Size (# AA): 414 amino acids
• Molecular Weight: 45kDa
• NCBI Gene Id: 23435
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: TAR DNA binding protein
• Description:
  View product citations for TARDBP Antibody - C-terminal region (ARP38942_T100) on CiteAb
• Alias Symbols: ALS10, TDP-43
• Peptide Sequence: Synthetic peptide located within the following region: MGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWG
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Buratti,E., (2005) J. Biol. Chem. 280 (45), 37572-37584
• Description of Target: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
• Protein Interactions: ZFAND2B; TARDBP; UBC; SUMO2; STAU1; SUMO3; RNF4; USP8; RBM45; RNF2; LSM6; UBE2E3; GNB2L1; MED6; RPA1; WWOX; RPA3; RPA2; PARK2; EED; BMI1; RBM39; BAG2; RPL23; DDX21; PRPF3; USP10; SART1; RPL14; H1FX; FUBP3; PABPC4; EIF4G3; EIF3I; EIF3H; EIF3C; EIF3B; EIF3A
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-TARDBP (ARP38942_T100) antibody is Catalog # AAP38942 (Previous Catalog # AAPP22968)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TARDBP
• Uniprot ID: Q13148
• Protein Name: TAR DNA-binding protein 43
• Publications:

  Bannister, J. P. et al. Ca(V)1.2 channel N-terminal splice variants modulate functional surface expression in resistance size artery smooth muscle cells. J. Biol. Chem. 286, 15058-66 (2011). 21358643

  Stress Granule Assembly Can Facilitate but Is Not Required for TDP-43 Cytoplasmic Aggregation. Biomolecules. 10, (2020). 32992901

• Sample Type Confirmation:

  TARDBP is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_031401
• Purification: Protein A purified
• Nucleotide Accession #: NM_007375
• Tested Species Reactivity: Human
• Gene Symbol: TARDBP
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%
• Image 1: Jurkat
  
  Host: Rabbit
  Target Name: TARDBP
  Sample Type: Jurkat
  Lane A: Primary Antibody
  Lane B: Primary Antibody + Blocking Peptide
  Primary Antibody Concentration: 1.25ug/mL
  Peptide Concentration: 1.0ug/mL
  Lysate Quantity: 25ug/lane
  Gel Concentration: 12%
• Image 2: Human Liver
  
  Human Liver
• Image 3: Human Heart
  
  Human Heart
• Image 4: Human Heart
  
  Rabbit Anti-TARDBP antibody
  Catalog Number: ARP38942
  Paraffin Embedded Tissue: Human Heart
  cell Cellular Data: cardiac cell
  Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X