STAT1 Antibody - N-terminal region (ARP38933_P050)

• Product Number: ARP38933_P050
• Name: STAT1 Antibody - N-terminal region (ARP38933_P050)
• Protein Size (# AA): 750 amino acids
• Molecular Weight: 87 kDa
• NCBI Gene Id: 6772
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Signal transducer and activator of transcription 1, 91kDa
• Alias Symbols: CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91
• Peptide Sequence: Synthetic peptide located within the following region: MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDV
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Battle,T.E., (2006) Cancer Res. 66 (7), 3649-3657
• Description of Target: STAT1 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
• Protein Interactions: HUWE1; ISG15; PIAS1; UBC; STAT1; EIF1AD; OTUD4; LMO2; GNB2; DTX3L; EGFR; NAA15; SNX6; CAND1; VPS29; GSPT2; JMJD6; RTF1; HSPA4L; ATG7; YAP1; PAWR; KARS; IDE; CTTN; VPS26A; HIRIP3; NAA10; TSNAX; TBCD; SNX2; SNX1; SHMT2; SHMT1; SARS; ATP6V1C1; APEH; USP13; E
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Additional Information: IHC Information: Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
• Blocking Peptide: For anti-STAT1 (ARP38933_P050) antibody is Catalog # AAP38933 (Previous Catalog # AAPY00742)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human STAT1
• Uniprot ID: P42224
• Protein Name: Signal transducer and activator of transcription 1-alpha/beta
• Publications:

  Bone marrow mesenchymal stromal cells with CD47 high expression via the signal transducer and activators of transcription signaling pathway preventing myocardial fibrosis. Int J Clin Exp Pathol. 8, 10555-64 (2015). 26617765

• Protein Accession #: NP_009330
• Purification: Affinity Purified
• Nucleotide Accession #: NM_007315
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: STAT1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 86%; Zebrafish: 86%
• Image 1: Mouse Pancreas
  
  Host: Mouse
  Target Name: STAT1
  Sample Tissue: Mouse Pancreas
  Antibody Dilution: 1ug/ml
• Image 2: r-STAT1
  
  WB Suggested Anti-STAT1 Antibody Titration: 1 ug/ml
  Positive Control: STAT1
• Image 3: Human Pancreas
  
  Immunohistochemistry with Pancreas tissue at an antibody concentration of 5.0ug/ml using anti-STAT1 antibody (ARP38933_P050)
• Image 4: Human Kidney
  
  Rabbit Anti-STAT1 Antibody
  Catalog Number: ARP38933
  Paraffin Embedded Tissue: Human Kidney
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X