NFYB Antibody - N-terminal region (ARP38748_P050)

• Product Number: ARP38748_P050
• Name: NFYB Antibody - N-terminal region (ARP38748_P050)
• Protein Size (# AA): 207 amino acids
• Molecular Weight: 23kDa
• Subunit: beta
• NCBI Gene Id: 4801
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Nuclear transcription factor Y, beta
• Alias Symbols: HAP3, CBF-A, CBF-B, NF-YB
• Peptide Sequence: Synthetic peptide located within the following region: MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKE
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Kimura,K., (2006) Genome Res. 16 (1), 55-65
• Description of Target: NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.
• Protein Interactions: AGTRAP; POLE4; DRAP1; NFYA; LYN; CDK2; CCNE1; CCNA2; TP53; SMYD3; NFYC; MAOB; UBC; CREBBP; SP1; HDAC1; EP300; KAT2B; TP73; TAF12; TAF11; TAF6; GRB2; YBX3; CEBPZ; C1QBP; TBP; CIITA; CNTN2; MYC; ELF1; YBX1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-NFYB (ARP38748_P050) antibody is Catalog # AAP38748 (Previous Catalog # AAPP20954)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NFYB
• Uniprot ID: P25208
• Protein Name: Nuclear transcription factor Y subunit beta
• Protein Accession #: NP_006157
• Purification: Affinity Purified
• Nucleotide Accession #: NM_006166
• Tested Species Reactivity: Human
• Gene Symbol: NFYB
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Transfected 293T
  
  WB Suggested Anti-NFYB Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: Transfected 293T