SMAD2 Antibody - N-terminal region (ARP38694_T100)

Data Sheet
 
Product Number ARP38694_T100
Product Page www.avivasysbio.com/smad2-antibody-n-terminal-region-arp38694-t100.html
Name SMAD2 Antibody - N-terminal region (ARP38694_T100)
Protein Size (# AA) 467 amino acids
Molecular Weight 51kDa
NCBI Gene Id 4087
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name SMAD family member 2
Alias Symbols JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2
Peptide Sequence Synthetic peptide located within the following region: VVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDE
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Zheng,X., (2006) EMBO J. 25 (3), 615-627
Description of Target SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
Protein Interactions HDAC2; UBC; SMAD4; SKIL; ANP32B; TUBA1B; SYT1; SOD1; ANP32E; PTMS; NEFM; JUN; ATP5I; RHOA; OTUB1; ZFYVE9; PPARG; TP53; TGFBR1; TRIM33; HCVgp1; TSC2; FHL3; FHL2; FHL1; CSNK1D; KLF5; SH2D2A; SKI; LCK; IRF7; IRF3; RNF111; LEMD3; PIK3CA; ST13; ST5; SRI; SPTBN
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-SMAD2 (ARP38694_T100) antibody
Blocking Peptide For anti-SMAD2 (ARP38694_T100) antibody is Catalog # AAP38694 (Previous Catalog # AAPP20883)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD2
Uniprot ID Q15796
Protein Name Mothers against decapentaplegic homolog 2
Protein Accession # NP_005892
Purification Protein A purified
Nucleotide Accession # NM_005901
Tested Species Reactivity Human, Rat
Gene Symbol SMAD2
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
Image 1
Human HepG2
WB Suggested Anti-SMAD2 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:1562500
Positive Control: HepG2 cell lysate
Image 2
transfected HEK-293
Lanes:
Lane1: 2ug HEK293lysate
Lane2: 2ug SMAD2 transfected HEK293 lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
SMAD2
Submitted by:
Dominique Alfandari, University of Massachusetts
Image 3
Rat Liver
Host: Rat
Target Name: SMAD2
Sample Tissue: Rat Liver
Antibody Dilution: 1ug/ml
 

AVIVA SYSTEMS BIOLOGY manufactures and sells quality antibody products covering genome wide proteins.

AVIVA SYSTEMS BIOLOGY
10211 Pacific Mesa Blvd, Ste 401, San Diego, CA 92121 USA | Tel: (858)552-6979 | info@avivasysbio.com