| Product Number |
ARP38690_P050 |
| Product Page |
www.avivasysbio.com/sap18-antibody-n-terminal-region-arp38690-p050.html |
| Name |
SAP18 Antibody - N-terminal region (ARP38690_P050) |
| Protein Size (# AA) |
153 amino acids |
| Molecular Weight |
18kDa |
| Subunit |
SAP18 |
| NCBI Gene Id |
10284 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Sin3A-associated protein, 18kDa |
| Alias Symbols |
SAP18P, 2HOR0202 |
| Peptide Sequence |
Synthetic peptide located within the following region: EIKKEPEKPIDREKTCPLLLRVFTTNNGRHHRMDEFSRGNVPSSELQIYT |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Zhang,Y., (1997) Cell 89 (3), 357-364 |
| Description of Target |
Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. SAP18 is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter.Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. |
| Protein Interactions |
INCA1; RBM39; UBC; RNF2; SUZ12; BMI1; CSNK2A2; MAGOH; EIF4A3; VCP; NFIA; SEPT2; MYO1C; IK; HNRNPU; HNRNPC; GTF3C1; SEPT7; CDC5L; U2AF1; SNRPD2; SMARCC2; SMARCB1; SRSF7; SRSF5; SRSF3; SRSF1; SAFB; S100A9; PSAP; NHP2L1; TPR; TFPI; SURF4; SSRP1; SSR3; LOC255 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-SAP18 (ARP38690_P050) antibody |
| Blocking Peptide |
For anti-SAP18 (ARP38690_P050) antibody is Catalog # AAP38690 (Previous Catalog # AAPP20879) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human SAP18 |
| Uniprot ID |
O00422 |
| Protein Name |
Histone deacetylase complex subunit SAP18 |
| Protein Accession # |
NP_005861 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_005870 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
SAP18 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93% |
| Image 1 | Transfected 293T
 | WB Suggested Anti-SAP18 Antibody Titration: 0.125ug/ml
ELISA Titer: 1:312500
Positive Control: Transfected 293T |
|
| Image 2 | Human testis tissue
 | Rabbit Anti-SAP18 Antibody
Catalog Number: ARP38690_P050
Formalin Fixed Paraffin Embedded Tissue: Human Testis Tissue
Observed Staining: Cytoplasm in spermatogonia and spermatocytes
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
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