| Product Number |
ARP38613_T100 |
| Product Page |
www.avivasysbio.com/nfil3-antibody-n-terminal-region-arp38613-t100.html |
| Name |
NFIL3 Antibody - N-terminal region (ARP38613_T100) |
| Protein Size (# AA) |
462 amino acids |
| Molecular Weight |
51kDa |
| NCBI Gene Id |
4783 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Nuclear factor, interleukin 3 regulated |
| Alias Symbols |
E4BP4, IL3BP1, NFIL3A, NF-IL3A |
| Peptide Sequence |
Synthetic peptide located within the following region: MQLRKMQTVKKEQASLDASSNVDKMMVLNSALTEVSEDSTTGEDVLLSEG |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Ozkurt,I.C., et al., (2003) J. Biol. Chem. 278 (29), 26803-26809 |
| Description of Target |
NFIL3 is a basic leucine zipper (bZIP) transcription factor that represses or activates transcription in non-osteoblastic cells. NFIL3 play a role in attenuation of PTH target gene transcription in osteoblasts. NFIL3 repression domain interacts specifically with the TBP binding repressor protein Dr1.Glucocorticoid-mediated upregulation of the bZIP transcriptional repressor gene, NFIL3, is dependent on [Ca2+]i levels, and correlates with Glucocorticoid-evoked apoptosis of Glucocorticoid-sensitive CEM-C7-14 cells. NFIL3 is involved in a distinct growth factor-regulated signaling pathway that is responsible for the survival of early B-cell progenitors, and whose alteration by E2A-HLF leads to childhood B lineage leukemia. |
| Protein Interactions |
AMOTL2; TRAF2; MAFF; BATF; NFIL3; MAFG; DDIT3; CBX8; SUMO2; UBC; CREB3; DR1; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-NFIL3 (ARP38613_T100) antibody |
| Blocking Peptide |
For anti-NFIL3 (ARP38613_T100) antibody is Catalog # AAP38613 (Previous Catalog # AAPP20804) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human NFIL3 |
| Uniprot ID |
Q14211 |
| Protein Name |
Nuclear factor interleukin-3-regulated protein |
| Protein Accession # |
NP_005375 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_005384 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
NFIL3 |
| Predicted Species Reactivity |
Human, Cow, Dog, Pig, Sheep |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 86%; Dog: 86%; Human: 100%; Pig: 79%; Sheep: 79% |
| Image 1 | Transfected 293T
 | WB Suggested Anti-NFIL3 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:312500
Positive Control: Transfected 293T |
|
| Image 2 | Human heart
 | Rabbit Anti-NFIL3 Antibody
Catalog Number: ARP38613_T100
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Nucleus
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
