SMARCE1 Antibody - N-terminal region (ARP38224_P050)

• Product Number: ARP38224_P050
• Name: SMARCE1 Antibody - N-terminal region (ARP38224_P050)
• Protein Size (# AA): 411 amino acids
• Molecular Weight: 47kDa
• NCBI Gene Id: 6605
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
• Alias Symbols: CSS5, BAF57
• Peptide Sequence: Synthetic peptide located within the following region: MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSR
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Chen,J. (2005) Mol. Cell. Biol. 25 (20), 9016-9027
• Description of Target: SMARCE1 is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.
• Protein Interactions: NOTCH2NL; KRTAP10-9; CCDC172; NBPF22P; TXLNA; MIPOL1; KRT40; MRFAP1L1; SYCE1; ING5; USHBP1; CEP70; CEP63; CCDC136; RINT1; TRIM54; AMOTL2; MED4; TFIP11; NUP62; MTUS2; EXOC7; RALBP1; SPAG5; JAKMIP2; TRIP10; STX11; CEP170P1; MEOX2; KRT31; KRT15; KIFC3; GOLGA
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-SMARCE1 (ARP38224_P050) antibody is Catalog # AAPS05009
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMARCE1
• Uniprot ID: Q969G3
• Protein Name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
• Sample Type Confirmation:

  SMARCE1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_003070
• Purification: Affinity Purified
• Nucleotide Accession #: NM_003079
• Tested Species Reactivity: Human
• Gene Symbol: SMARCE1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Image 1: Human kidney
  
  Human kidney
• Image 2: Human Jurkat
  
  WB Suggested Anti-SMARCE1 Antibody Titration: 0.2-1 ug/ml
  Positive Control: Jurkat cell lysateSMARCE1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
• Image 3: Human, Mouse, Rat
  
  Lane 1: HeLa (human)Lane 2: NHEM (human)Lane 3: Melba (mouse)Lane 4: NIH3T3 (mouse)Lane 5: S16 (rat)Lane 6: H9C2 (rat)
  Primary Antibody Dilution:
  1:500
  Secondary Antibody:
  Donkey anti-rabbit-HRP
  Secondary Antibody Dilution:
  1:5000
  Gene Name:
  SMARCE1
  Submitted by:
  Ivana de la Serna, University of Toledo
• Image 4: Colon
  
  Rabbit Anti-SMARCE1 antibody
  Catalog Number: ARP38224
  Formalin Fixed Paraffin Embedded Tissue: Human Adult Colon
  Observed Staining: Cytoplasm in hepatocytes
  Primary Antibody Concentration: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 – 2.0 sec
  
• Image 5: Human Lung
  
  Rabbit Anti-SMARCE1 antibody
  Catalog Number: ARP38224
  Paraffin Embedded Tissue: Human Lung
  cell Cellular Data: alveolar cell
  Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X