Product Number |
ARP38224_P050 |
Product Page |
www.avivasysbio.com/smarce1-antibody-n-terminal-region-arp38224-p050.html |
Name |
SMARCE1 Antibody - N-terminal region (ARP38224_P050) |
Protein Size (# AA) |
411 amino acids |
Molecular Weight |
47kDa |
NCBI Gene Id |
6605 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 |
Alias Symbols |
CSS5, BAF57 |
Peptide Sequence |
Synthetic peptide located within the following region: MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSR |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Chen,J. (2005) Mol. Cell. Biol. 25 (20), 9016-9027 |
Description of Target |
SMARCE1 is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. |
Protein Interactions |
NOTCH2NL; KRTAP10-9; CCDC172; NBPF22P; TXLNA; MIPOL1; KRT40; MRFAP1L1; SYCE1; ING5; USHBP1; CEP70; CEP63; CCDC136; RINT1; TRIM54; AMOTL2; MED4; TFIP11; NUP62; MTUS2; EXOC7; RALBP1; SPAG5; JAKMIP2; TRIP10; STX11; CEP170P1; MEOX2; KRT31; KRT15; KIFC3; GOLGA |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-SMARCE1 (ARP38224_P050) antibody |
Blocking Peptide |
For anti-SMARCE1 (ARP38224_P050) antibody is Catalog # AAPS05009 |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human SMARCE1 |
Uniprot ID |
Q969G3 |
Protein Name |
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 |
Sample Type Confirmation |
SMARCE1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat |
Protein Accession # |
NP_003070 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_003079 |
Tested Species Reactivity |
Human |
Gene Symbol |
SMARCE1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% |
Image 1 | Human kidney
| Human kidney |
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Image 2 | Human Jurkat
| WB Suggested Anti-SMARCE1 Antibody Titration: 0.2-1 ug/ml Positive Control: Jurkat cell lysateSMARCE1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells |
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Image 3 | Human, Mouse, Rat
| Lane 1: HeLa (human)Lane 2: NHEM (human)Lane 3: Melba (mouse)Lane 4: NIH3T3 (mouse)Lane 5: S16 (rat)Lane 6: H9C2 (rat) Primary Antibody Dilution: 1:500 Secondary Antibody: Donkey anti-rabbit-HRP Secondary Antibody Dilution: 1:5000 Gene Name: SMARCE1 Submitted by: Ivana de la Serna, University of Toledo |
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Image 4 | Colon
| Rabbit Anti-SMARCE1 antibody Catalog Number: ARP38224 Formalin Fixed Paraffin Embedded Tissue: Human Adult Colon Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 2.0 sec
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Image 5 | Human Lung
| Rabbit Anti-SMARCE1 antibody
Catalog Number: ARP38224
Paraffin Embedded Tissue: Human Lung
cell Cellular Data: alveolar cell
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
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