SIAH1 Antibody - C-terminal region (ARP38212_T100)

• Product Number: ARP38212_T100
• Name: SIAH1 Antibody - C-terminal region (ARP38212_T100)
• Protein Size (# AA): 282 amino acids
• Molecular Weight: 31kDa
• NCBI Gene Id: 6477
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Siah E3 ubiquitin protein ligase 1
• Alias Symbols: SIAH1A
• Peptide Sequence: Synthetic peptide located within the following region: LVLEKQEKYDGHQQFFAIVQLIGTRKQAENFAYRLELNGHRRRLTWEATP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Avraham,E., et al., (2005) J. Biol. Chem. 280 (52), 42877-42886
• Description of Target: SIAH1 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterizedThis gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
• Protein Interactions: MX1; MAB21L1; KIFC3; KCNJ10; INHA; UBE2K; FLI1; EEF1D; PHC2; PHC1; CDC34; CAPG; BCL6; TRIM23; AQP1; PFKM; ZCCHC13; MTIF3; UPP2; MOB3C; SPATA4; PRR20A; HIST4H4; EXOC3-AS1; PTPMT1; RPS19BP1; SPATC1L; KATNAL1; TRIM7; GDPD5; ZBP1; KIAA1683; ARMC9; KIAA0319L;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: IHC Information: Paraffin embedded livertissue, tested with an antibody dilution of 5 ug/ml.
• Blocking Peptide: For anti-SIAH1 (ARP38212_T100) antibody is Catalog # AAP38212 (Previous Catalog # AAPP20388)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SIAH1
• Uniprot ID: Q8IUQ4
• Protein Name: E3 ubiquitin-protein ligase SIAH1
• Sample Type Confirmation:

  SIAH1 is strongly supported by BioGPS gene expression data to be expressed in HEK293T

• Protein Accession #: NP_003022
• Purification: Protein A purified
• Nucleotide Accession #: NM_003031
• Tested Species Reactivity: Human
• Gene Symbol: SIAH1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Image 1: Human HEK293T
  
  Lanes:
  Lane1: 50 ug human HEK-293T lysate
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Anti-rabbit-IgG
  Secondary Antibody Dilution:
  1:5000
  Gene Name:
  SIAH1
  Submitted by:
  Peter Brand & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University Jena
  SIAH1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells
• Image 2: Human Liver
  
  Liver
• Image 3: Human Placenta
  
  WB Suggested Anti-SIAH1 Antibody Titration: 1.25ug/ml
  ELISA Titer: 1:312500
  Positive Control: Human Placenta
• Image 4: Mouse Brain, Mouse Pancreas
  
  Host: Rabbit
  Target: SIAH1
  Positive control (+): Mouse Brain (M-BR)
  Negative control (-): Mouse Pancreas (M-PA)
  Antibody concentration: 1ug/ml