||SIAH1 antibody - C-terminal region (ARP38212_T100)
|Protein Size (# AA)
||282 amino acids
||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
|NCBI Gene Id
||Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
|Official Gene Full Name
||Siah E3 ubiquitin protein ligase 1
||This is a rabbit polyclonal antibody against SIAH1. It was validated on Western Blot using a cell lysate as a positive control. Aviva Systems Biology strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (firstname.lastname@example.org).
||Synthetic peptide located within the following region: LVLEKQEKYDGHQQFFAIVQLIGTRKQAENFAYRLELNGHRRRLTWEATP
||Avraham,E., et al., (2005) J. Biol. Chem. 280 (52), 42877-42886
|Description of Target
||SIAH1 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterizedThis gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
||MX1; MAB21L1; KIFC3; KCNJ10; INHA; UBE2K; FLI1; EEF1D; PHC2; PHC1; CDC34; CAPG; BCL6; TRIM23; AQP1; PFKM; ZCCHC13; MTIF3; UPP2; MOB3C; SPATA4; PRR20A; HIST4H4; EXOC3-AS1; PTPMT1; RPS19BP1; SPATC1L; KATNAL1; TRIM7; GDPD5; ZBP1; KIAA1683; ARMC9; KIAA0319L;
|Reconstitution and Storage
||For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
||IHC Information: Paraffin embedded livertissue, tested with an antibody dilution of 5 ug/ml.
||Domestic: within 1-2 days delivery International: 1-2 days
||For anti-SIAH1 (ARP38212_T100) antibody is Catalog # AAP38212 (Previous Catalog # AAPP20388)
||The immunogen is a synthetic peptide directed towards the C terminal region of human SIAH1
|Complete computational species homology data
||Find tissues and cell lines supported by DNA array analysis to express SIAH1.
||E3 ubiquitin-protein ligase SIAH1
|Sample Type Confirmation
SIAH1 is strongly supported by BioGPS gene expression data to be expressed in HEK293T
|Protein Accession #
||Protein A purified
||Find tissues and cell lines supported by RNA-seq analysis to express SIAH1.
|Nucleotide Accession #
||This antibody may replace item sc-22765 from Santa Cruz Biotechnology.
||sc-22765; sc-37495; sc-44102; sc-5504; sc-5505; sc-5506; sc-81785; sc-81786; sc-81787; sc-81788
||Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
|Predicted Homology Based on Immunogen Sequence
||Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
|Human Placenta||WB Suggested Anti-SIAH1 Antibody Titration: 1.25ug/ml|
ELISA Titer: 1:312500
Positive Control: Human Placenta
|Human HEK293T||Lanes: |
Lane1: 50 ug human HEK-293T lysate
Primary Antibody Dilution:
Secondary Antibody Dilution:
Peter Brand & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University Jena
SIAH1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells