Product Number |
ARP38031_T100 |
Product Page |
www.avivasysbio.com/eno1-antibody-c-terminal-region-arp38031-t100.html |
Name |
ENO1 Antibody - C-terminal region (ARP38031_T100) |
Protein Size (# AA) |
434 amino acids |
Molecular Weight |
47 kDa |
NCBI Gene Id |
2023 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Enolase 1, (alpha) |
Alias Symbols |
NNE, PPH, MPB1, ENO1L1, HEL-S-17 |
Peptide Sequence |
Synthetic peptide located within the following region: MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELR |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Ghosh,A.K., et al., (2005) J. Biol. Chem. 280 (14), 14325-14330 |
Description of Target |
ENO1 is one of three enolase isoenzymes found in mammals; its gene encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. |
Protein Interactions |
UBC; FUS; HUWE1; ISG15; SUMO2; SUMO3; IVNS1ABP; STAU1; MDM2; WWOX; EED; FBXO6; UPF2; YWHAQ; UBD; TERT; SRC; RAD52; NPM1; BHLHE40; VCAM1; HCVgp1; ITGA4; FN1; ATF2; SERPING1; AMBP; LIG4; BRCA1; MDC1; PGK1; ITPA; GAPDH; FYN; FLNC; ENO3; Htt; TPI1; PPIA; AKT1 |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-ENO1 (ARP38031_T100) antibody |
Blocking Peptide |
For anti-ENO1 (ARP38031_T100) antibody is Catalog # AAP38031 (Previous Catalog # AAPP10016) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human ENO1 |
Uniprot ID |
Q53FT9 |
Protein Name |
Alpha-enolase |
Sample Type Confirmation |
ENO1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat |
Protein Accession # |
NP_001419 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_001428 |
Tested Species Reactivity |
Human |
Gene Symbol |
ENO1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Guinea Pig, Rabbit, Sheep, Zebrafish |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100% |
Image 1 |
| 25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.
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