ENO1 Antibody - C-terminal region (ARP38031_T100)

Data Sheet
 
Product Number ARP38031_T100
Product Page www.avivasysbio.com/eno1-antibody-c-terminal-region-arp38031-t100.html
Name ENO1 Antibody - C-terminal region (ARP38031_T100)
Protein Size (# AA) 434 amino acids
Molecular Weight 47 kDa
NCBI Gene Id 2023
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Enolase 1, (alpha)
Alias Symbols NNE, PPH, MPB1, ENO1L1, HEL-S-17
Peptide Sequence Synthetic peptide located within the following region: MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELR
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Ghosh,A.K., et al., (2005) J. Biol. Chem. 280 (14), 14325-14330
Description of Target ENO1 is one of three enolase isoenzymes found in mammals; its gene encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
Protein Interactions UBC; FUS; HUWE1; ISG15; SUMO2; SUMO3; IVNS1ABP; STAU1; MDM2; WWOX; EED; FBXO6; UPF2; YWHAQ; UBD; TERT; SRC; RAD52; NPM1; BHLHE40; VCAM1; HCVgp1; ITGA4; FN1; ATF2; SERPING1; AMBP; LIG4; BRCA1; MDC1; PGK1; ITPA; GAPDH; FYN; FLNC; ENO3; Htt; TPI1; PPIA; AKT1
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-ENO1 (ARP38031_T100) antibody
Blocking Peptide For anti-ENO1 (ARP38031_T100) antibody is Catalog # AAP38031 (Previous Catalog # AAPP10016)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human ENO1
Uniprot ID Q53FT9
Protein Name Alpha-enolase
Sample Type Confirmation

ENO1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

Protein Accession # NP_001419
Purification Protein A purified
Nucleotide Accession # NM_001428
Tested Species Reactivity Human
Gene Symbol ENO1
Predicted Species Reactivity Human, Mouse, Rat, Guinea Pig, Rabbit, Sheep, Zebrafish
Application WB
Predicted Homology Based on Immunogen Sequence Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
Image 1

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.
 

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