| Product Number |
ARP37997_T100 |
| Product Page |
www.avivasysbio.com/twist1-antibody-c-terminal-region-arp37997-t100.html |
| Name |
TWIST1 Antibody - C-terminal region (ARP37997_T100) |
| Protein Size (# AA) |
202 amino acids |
| Molecular Weight |
21kDa |
| NCBI Gene Id |
7291 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Twist homolog 1 (Drosophila) |
| Description |
|
| Alias Symbols |
CRS, CSO, SCS, ACS3, CRS1, BPES2, BPES3, SWCOS, TWIST, bHLHa38 |
| Peptide Sequence |
Synthetic peptide located within the following region: DKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVW |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Reinhold,M.I., et al., (2006) J. Biol. Chem. 281 (3), 1381-1388 |
| Description of Target |
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation.TWIST1 is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. |
| Protein Interactions |
UBC; HOXA5; ETS2; TP53; KAT2B; SETD8; HES6; MTA2; BRAP; TCF3; RELA; PPP2CA; HDAC2; CHD3; TWIST2; ELSPBP1; WDR5; HDAC3; RBBP7; CHD4; SOX2; EP300; TWIST1; GLI3; MYOD1; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-TWIST1 (ARP37997_T100) antibody |
| Additional Information |
IHC Information: Paraffin embedded uterus tissue, tested with an antibody dilution of 5 ug/ml. |
| Blocking Peptide |
For anti-TWIST1 (ARP37997_T100) antibody is Catalog # AAP37997 (Previous Catalog # AAPP20171) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human TWIST1 |
| Uniprot ID |
Q15672 |
| Protein Name |
Twist-related protein 1 |
| Publications |
Guha, P. et al. Nicotine promotes apoptosis resistance of breast cancer cells and enrichment of side population cells with cancer stem cell-like properties via a signaling cascade involving galectin-3, a9 nicotinic acetylcholine receptor and STAT3. Breast Cancer Res. Treat. 145, 5-22 (2014). 24668500
Phosphorylation of ETS1 by Src family kinases prevents its recognition by the COP1 tumor suppressor. Cancer Cell. 26, 222-34 (2014). 25117710 |
| Protein Accession # |
NP_000465 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_000474 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
TWIST1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 79%; Guinea Pig: 79%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 79%; Rat: 100%; Zebrafish: 100% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-TWIST1 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate |
|
| Image 2 | Human Uterus
 | Uterus |
|
| Image 3 | Jurkat
 | Host: Rabbit
Target Name: TWIST1
Sample Type: Jurkat
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 2.5ug/mL
Peptide Concentration: 2.0ug/mL
Lysate Quantity: 25ug/lane
Gel Concentration: 12% |
|
| Image 4 | A549, U937
 | Host: Rabbit
Target: TWIST1
Positive control (+): A549 (N03)
Negative control (-): U937 (N31)
Antibody concentration: 1ug/ml |
|
