| Product Number |
ARP37977_T100 |
| Product Page |
www.avivasysbio.com/mitf-antibody-n-terminal-region-arp37977-t100.html |
| Name |
MITF Antibody - N-terminal region (ARP37977_T100) |
| Protein Size (# AA) |
419 amino acids |
| Molecular Weight |
47kDa |
| NCBI Gene Id |
4286 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Microphthalmia-associated transcription factor |
| Alias Symbols |
MI, WS2, CMM8, WS2A, COMMAD, bHLHe32 |
| Peptide Sequence |
Synthetic peptide located within the following region: THLENPTKYHIQQAQRQQVKQYLSTTLANKHANQVLSLPCPNQPGDHVMP |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Park,H.Y., et al., (2006) Biochem. J. 395 (3), 571-578 |
| Description of Target |
MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| Protein Interactions |
EBP; STUB1; HK3; EPAS1; LEF1; APP; HINT1; ELAVL1; CDK2; SUMO2; PSMD14; MARK3; PIAS3; PIAS2; PIAS1; SUMO1; UBE2I; PAX3; OTX2; PAX6; PATZ1; GSK3B; TFEC; EP300; RPS6KA1; TFEB; MAPK14; TFE3; MAPK1; SPI1; FOS; CTNNB1; CDKN1A; RB1; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-MITF (ARP37977_T100) antibody |
| Blocking Peptide |
For anti-MITF (ARP37977_T100) antibody is Catalog # AAP37977 (Previous Catalog # AAPP20080) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human MITF |
| Uniprot ID |
O75030-9 |
| Protein Name |
Microphthalmia-associated transcription factor |
| Protein Accession # |
NP_000239 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_000248 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
MITF |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100% |
| Image 1 | Mouse Pancreas
 | Host: Mouse
Target Name: MITF
Sample Tissue: Mouse Pancreas
Antibody Dilution: 1ug/ml |
|
| Image 2 | Human HepG2
 | WB Suggested Anti-MITF Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1562500
Positive Control: HepG2 cell lysate |
|
