| Product Number |
ARP37465_P050 |
| Product Page |
www.avivasysbio.com/nfat5-antibody-middle-region-arp37465-p050.html |
| Name |
NFAT5 Antibody - middle region (ARP37465_P050) |
| Protein Size (# AA) |
1534 amino acids |
| Molecular Weight |
166kDa |
| NCBI Gene Id |
54446 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Nuclear factor of activated T cells 5 |
| Alias Symbols |
OR, Ton, nfa, CAG-8, CAG80, OREBP, nfatz, NFATL1, TonEBP, AI225870, mKIAA0827, B130038B15Rik |
| Peptide Sequence |
Synthetic peptide located within the following region: TIQTQDISQPGTFPAVSAASQLPSSDALLQQATQFQTREAQSRDTIQSDT |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Pan,S., et al., (2000) Biochem. Biophys. Res. Commun. 272 (3), 765-776 |
| Description of Target |
Nfat5 contains 1 RHD (Rel-like) domain, and plays a role in the inducible expression of genes. Nfat5 regulates hypertonicity-induced cellular accumulation of osmolytes. Nfat5 might be capable of forming stable dimers with DNA elements |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-NFAT5 (ARP37465_P050) antibody |
| Blocking Peptide |
For anti-NFAT5 (ARP37465_P050) antibody is Catalog # AAP37465 (Previous Catalog # AAPP09821) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of mouse NFAT5 |
| Uniprot ID |
Q9WV30 |
| Protein Name |
NFATz2 EMBL AAF79117.1 |
| Protein Accession # |
AAF79117 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_018823 |
| Tested Species Reactivity |
Mouse |
| Gene Symbol |
NFAT5 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 100%; Guinea Pig: 83%; Horse: 93%; Human: 93%; Mouse: 100%; Pig: 93%; Rabbit: 86%; Rat: 93% |
| Image 1 | Mouse SP2/0
 | WB Suggested Anti-NFAT5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: SP2/0 cell lysate |
|
| Image 2 | HT-29
 | Lanes:
Lane 1: 20ug HT 29 lysate blocked with 5% FBS Lane 2: 20ug HT 29 lysate blocked with no FBS Lane 3: 20ug HT-29 lyaste blocked with no PBS + 100 mM NaCl
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-rabbit-HRP
Secondary Antibody Dilution:
1:1000
Gene Name:
NFAT5
Submitted by:
Manuel Fresno, CBMSO
|
|
| Image 3 | Mouse Skeletal Muscle
 | Host: Rabbit
Target Name: Nfat5
Sample Tissue: Mouse Skeletal Muscle
Antibody Dilution: 3ug/ml |
|
