FOXH1 Antibody - middle region (ARP36853_P050)

• Product Number: ARP36853_P050
• Name: FOXH1 Antibody - middle region (ARP36853_P050)
• Protein Size (# AA): 401 amino acids
• Molecular Weight: 39 kDa
• NCBI Gene Id: 14106
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Forkhead box H1
• Alias Symbols: Fas, Fast1, Fast2, fast-1, fast-2
• Peptide Sequence: Synthetic peptide located within the following region: GPSSSSETPLWPLCSLPGPTIIEGESSQGEVIRPSPVTPDQGSWPLHLLE
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Georgiades,P. (2006) Development 133 (6), 1059-1068
• Description of Target: Foxh1 is a transcriptional activator. Foxh1 can recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3' and is required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Foxh1 forms a transcriptionally active complex containing FAST-1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).
• Protein Interactions: Nkx2-5; Gsc; Smad2; Gtf2a1; Rxrg; Smad3; Alx4; Mef2c;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-FOXH1 (ARP36853_P050) antibody is Catalog # AAP36853 (Previous Catalog # AAPY00604)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of mouse FOXH1
• Uniprot ID: O88621
• Protein Name: Forkhead box protein H1
• Protein Accession #: NP_032015
• Purification: Affinity Purified
• Nucleotide Accession #: NM_007989
• Tested Species Reactivity: Mouse
• Gene Symbol: FOXH1
• Predicted Species Reactivity: Mouse, Rat
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Mouse: 100%; Rat: 92%
• Image 1: Mouse NIH3T3 Whole Cell
  
  Host: Rabbit
  Target Name: FOXH1
  Sample Tissue: Mouse NIH3T3 Whole Cell
  Antibody Dilution: 1ug/ml
• Image 2: Mouse Mouse Thymus
  
  Host: Rabbit
  Target Name: Foxh1
  Sample Tissue: Mouse Mouse Thymus
  Antibody Dilution: 1ug/ml
• Image 3: Western Blot
  
  25 ug of the indicated Mouse whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.