| Product Number |
ARP36677_T100 |
| Product Page |
www.avivasysbio.com/spp1-antibody-c-terminal-region-arp36677-t100.html |
| Name |
SPP1 Antibody - C-terminal region (ARP36677_T100) |
| Protein Size (# AA) |
300 amino acids |
| Molecular Weight |
33kDa |
| NCBI Gene Id |
6696 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Secreted phosphoprotein 1 |
| Alias Symbols |
OPN, BNSP, BSPI, ETA-1 |
| Peptide Sequence |
Synthetic peptide located within the following region: DSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASS |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Iwata,M., et al., (2004) Blood 103 (12), 4496-4502 |
| Description of Target |
The SPP1 gene encodes an acidic matrix protein, mainly expressed in mineralized tissues, kidney and atherosclerotic vessels. This protein also contributes to several steps in the process of prostate carcinogenesis and metastasis. |
| Protein Interactions |
SGTA; MEOX2; PARP1; BAG6; PEPD; APOA1; UBQLN4; CISD2; TUBB; CC2D1B; COMMD1; RIMS4; C2orf50; CMTM3; DSEL; ZNF804A; USP42; TMEM168; LRRC4; GRIPAP1; TMEM30A; AMOTL2; C1RL; DNTTIP2; KCNIP1; STK39; EIF3K; ZNF638; AFF4; CACNG4; SLC39A6; PLD3; SNAPIN; NCSTN; CEP |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-SPP1 (ARP36677_T100) antibody |
| Blocking Peptide |
For anti-SPP1 (ARP36677_T100) antibody is Catalog # AAP36677 (Previous Catalog # AAPP08104) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human SPP1 |
| Uniprot ID |
P10451 |
| Protein Name |
Osteopontin |
| Protein Accession # |
NP_000573 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_000582 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
SPP1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Sheep |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 86%; Dog: 79%; Goat: 79%; Guinea Pig: 86%; Human: 100%; Mouse: 86%; Rat: 79%; Sheep: 79% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-SPP1 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate |
|
| Image 2 | Human kidney
 | Human kidney |
|
| Image 3 | Jurkat
 | Host: Rabbit
Target Name: SPP1
Sample Type: Jurkat
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 2.5ug/mL
Peptide Concentration: 2.0ug/mL
Lysate Quantity: 25ug/lane
Gel Concentration: 12% |
|
| Image 4 | Human Jurkat Whole Cell
 | Host: Rabbit
Target Name: SPP1
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 5 ug/ml |
|
