Product Number |
ARP36649_T100 |
Product Page |
www.avivasysbio.com/mcm4-antibody-middle-region-arp36649-t100.html |
Name |
MCM4 Antibody - middle region (ARP36649_T100) |
Protein Size (# AA) |
863 amino acids |
Molecular Weight |
95kDa |
NCBI Gene Id |
4173 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Minichromosome maintenance complex component 4 |
Alias Symbols |
NKCD, CDC21, CDC54, IMD54, NKGCD, hCdc21, P1-CDC21 |
Peptide Sequence |
Synthetic peptide located within the following region: VYKTHIDVIHYRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYE |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Ishimi,Y., et al., (2003) J. Biol. Chem. 278 (27), 24644-24650 |
Description of Target |
The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. The MCM4 gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. |
Protein Interactions |
HUWE1; UBC; PCNA; SUMO2; FKBP5; MCMBP; MDM2; POLK; CLUH; NCKAP1; CAP1; CAP2; EIF3B; MCM2; KPNA3; PRMT1; FKBP4; MCM7; MCM6; FBXW4; FBXO46; ITGA4; CDC5L; gag-pol; ORC6; CCNA1; VCAM1; DBF4; CDC7; LIG4; MCM5; MCM3; DCTN2; SF3A1; UBD; NEDD8; TONSL; SIRT7; ASF1 |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-MCM4 (ARP36649_T100) antibody |
Blocking Peptide |
For anti-MCM4 (ARP36649_T100) antibody is Catalog # AAP36649 (Previous Catalog # AAPP07906) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human MCM4 |
Uniprot ID |
P33991 |
Protein Name |
DNA replication licensing factor MCM4 |
Sample Type Confirmation |
MCM4 is strongly supported by BioGPS gene expression data to be expressed in Jurkat |
Protein Accession # |
NP_005905 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_005914 |
Tested Species Reactivity |
Human |
Gene Symbol |
MCM4 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 85% |
Image 1 | Human heart
| Rabbit Anti-MCM4 Antibody Catalog Number: ARP36649_T100 Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Nucleus Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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Image 2 | Human Jurkat
| WB Suggested Anti-MCM4 Antibody Titration: 1.25ug/ml ELISA Titer: 1:12500 Positive Control: Jurkat cell lysateMCM4 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells |
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