MCM4 Antibody - middle region (ARP36649_T100)

Data Sheet
 
Product Number ARP36649_T100
Product Page www.avivasysbio.com/mcm4-antibody-middle-region-arp36649-t100.html
Name MCM4 Antibody - middle region (ARP36649_T100)
Protein Size (# AA) 863 amino acids
Molecular Weight 95kDa
NCBI Gene Id 4173
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Minichromosome maintenance complex component 4
Alias Symbols NKCD, CDC21, CDC54, IMD54, NKGCD, hCdc21, P1-CDC21
Peptide Sequence Synthetic peptide located within the following region: VYKTHIDVIHYRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYE
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Ishimi,Y., et al., (2003) J. Biol. Chem. 278 (27), 24644-24650
Description of Target The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. The MCM4 gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks.
Protein Interactions HUWE1; UBC; PCNA; SUMO2; FKBP5; MCMBP; MDM2; POLK; CLUH; NCKAP1; CAP1; CAP2; EIF3B; MCM2; KPNA3; PRMT1; FKBP4; MCM7; MCM6; FBXW4; FBXO46; ITGA4; CDC5L; gag-pol; ORC6; CCNA1; VCAM1; DBF4; CDC7; LIG4; MCM5; MCM3; DCTN2; SF3A1; UBD; NEDD8; TONSL; SIRT7; ASF1
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-MCM4 (ARP36649_T100) antibody
Blocking Peptide For anti-MCM4 (ARP36649_T100) antibody is Catalog # AAP36649 (Previous Catalog # AAPP07906)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human MCM4
Uniprot ID P33991
Protein Name DNA replication licensing factor MCM4
Sample Type Confirmation

MCM4 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

Protein Accession # NP_005905
Purification Protein A purified
Nucleotide Accession # NM_005914
Tested Species Reactivity Human
Gene Symbol MCM4
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 85%
Image 1
Human heart
Rabbit Anti-MCM4 Antibody
Catalog Number: ARP36649_T100
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Nucleus
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 2
Human Jurkat
WB Suggested Anti-MCM4 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:12500
Positive Control: Jurkat cell lysateMCM4 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
 

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