| Product Number |
ARP36645_P050 |
| Product Page |
www.avivasysbio.com/mcm3-antibody-c-terminal-region-arp36645-p050.html |
| Name |
MCM3 Antibody - C-terminal region (ARP36645_P050) |
| Protein Size (# AA) |
808 amino acids |
| Molecular Weight |
89kDa |
| NCBI Gene Id |
4172 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Minichromosome maintenance complex component 3 |
| Alias Symbols |
HCC5, P1.h, RLFB, P1-MCM3 |
| Peptide Sequence |
Synthetic peptide located within the following region: SQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQVHTPKTADSQE |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Shukla,A., (2005) Biochem. Biophys. Res. Commun. 329 (4), 1240-1245 |
| Description of Target |
MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. |
| Protein Interactions |
SUMO2; MCMBP; UBC; KEAP1; MDM2; MCM6; MCM5; ACTBL2; MGEA5; CAP1; SAE1; HSP90B1; TPM3; HSP90AA1; FKBP5; FKBP4; MIB1; YWHAQ; UBD; TCEA1; MCM10; ORC5; ORC4; MCM2; ITGA4; FN1; CSNK2A1; CDK2; CDC6; CDC5L; PNKP; CDC7; VCAM1; NOTCH1; POLD1; POLA1; MCM7; PIPSL; P |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-MCM3 (ARP36645_P050) antibody |
| Blocking Peptide |
For anti-MCM3 (ARP36645_P050) antibody is Catalog # AAP36645 (Previous Catalog # AAPS06307) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human MCM3 |
| Uniprot ID |
P25205 |
| Protein Name |
DNA replication licensing factor MCM3 |
| Sample Type Confirmation |
MCM3 is supported by BioGPS gene expression data to be expressed in Jurkat |
| Protein Accession # |
NP_002379 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_002388 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
MCM3 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 92%; Dog: 93%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 92%; Pig: 100%; Rabbit: 93%; Rat: 100% |
| Image 1 | Human Lung
 | Human Lung |
|
| Image 2 | Human Jurkat
 | WB Suggested Anti-MCM3 Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat cell lysateMCM3 is supported by BioGPS gene expression data to be expressed in Jurkat |
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