Product Number |
ARP36598_P050 |
Product Page |
www.avivasysbio.com/gja1-antibody-n-terminal-region-arp36598-p050.html |
Name |
GJA1 Antibody - N-terminal region (ARP36598_P050) |
Protein Size (# AA) |
382 amino acids |
Molecular Weight |
42kDa |
NCBI Gene Id |
2697 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Gap junction protein, alpha 1, 43kDa |
Alias Symbols |
HSS, CMDR, CX43, EKVP, GJAL, ODDD, AVSD3, EKVP3, HLHS1, PPKCA |
Peptide Sequence |
Synthetic peptide located within the following region: LYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIE |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Gellhaus,A., et al., (2004) J. Biol. Chem. 279 (35), 36931-36942 |
Description of Target |
Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. |
Protein Interactions |
UBC; STAMBP; EPS15; UBQLN4; PSMD4; PSMD2; S100A1; GJA1; CNST; NEDD4; TJP1; HGS; TSG101; SGSM3; GJA5; MAPK7; MAPK3; CAV1; CSNK1D; SRC; PRKCG; PRKCE; PRKCB; PRKCA; MAPK1; NOV; CSK; GJA3; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-GJA1 (ARP36598_P050) antibody |
Blocking Peptide |
For anti-GJA1 (ARP36598_P050) antibody is Catalog # AAP36598 (Previous Catalog # AAPP07837) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human GJA1 |
Uniprot ID |
Q53FJ6 |
Protein Name |
Gap junction protein RuleBase RU000630 |
Protein Accession # |
NP_000156 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_000165 |
Tested Species Reactivity |
Human, Rat |
Gene Symbol |
GJA1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 92% |
Image 1 | rat stiatum
| WB Suggested Anti-GJA1 Antibody Positive Control: Lane1: 60ug rat stiatum Primary Antibody Dilution : 1:1000 Secondary Antibody : Goat anti-rabbit-IRDye800 Secondry Antibody Dilution : 1:10,000 Submitted by: Ruben van Vugt, The Nijmegen Centre for Molecular Life Sciences (NCMLS) |
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Image 2 | Rat
| Species+tissue/cell type: Total rat cardiac lysate 1: 8 ug total cardiac lysate 2: 15 ug total cardiac lysate 3: 30 ug total cardiac lysate 4: 50 ug total cardiac lysatePrimary antibody dilution: 0.25 ug/ml |
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Image 3 | Rat cardiac
| Lanes: Lane1: 8 ug total cardiac lysate Lane2: 15 ug total cardiac lysate Lane3: 30 ug total cardiac lysate Lane4: 50 ug total cardiac lysate Primary Antibody Dilution: 0.25 ug/ml Secondary Antibody:
Secondary Antibody Dilution:
Gene Name: GJA1 Submitted by: Anonymous
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Image 4 | Human Placenta
| WB Suggested Anti-GJA1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: Human Placenta |
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