GJA1 Antibody - N-terminal region (ARP36598_P050)

Data Sheet
 
Product Number ARP36598_P050
Product Page www.avivasysbio.com/gja1-antibody-n-terminal-region-arp36598-p050.html
Name GJA1 Antibody - N-terminal region (ARP36598_P050)
Protein Size (# AA) 382 amino acids
Molecular Weight 42kDa
NCBI Gene Id 2697
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Gap junction protein, alpha 1, 43kDa
Alias Symbols HSS, CMDR, CX43, EKVP, GJAL, ODDD, AVSD3, EKVP3, HLHS1, PPKCA
Peptide Sequence Synthetic peptide located within the following region: LYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIE
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Gellhaus,A., et al., (2004) J. Biol. Chem. 279 (35), 36931-36942
Description of Target Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
Protein Interactions UBC; STAMBP; EPS15; UBQLN4; PSMD4; PSMD2; S100A1; GJA1; CNST; NEDD4; TJP1; HGS; TSG101; SGSM3; GJA5; MAPK7; MAPK3; CAV1; CSNK1D; SRC; PRKCG; PRKCE; PRKCB; PRKCA; MAPK1; NOV; CSK; GJA3;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-GJA1 (ARP36598_P050) antibody
Blocking Peptide For anti-GJA1 (ARP36598_P050) antibody is Catalog # AAP36598 (Previous Catalog # AAPP07837)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human GJA1
Uniprot ID Q53FJ6
Protein Name Gap junction protein RuleBase RU000630
Protein Accession # NP_000156
Purification Affinity Purified
Nucleotide Accession # NM_000165
Tested Species Reactivity Human, Rat
Gene Symbol GJA1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 92%
Image 1
rat stiatum
WB Suggested Anti-GJA1 Antibody
Positive Control: Lane1: 60ug rat stiatum
Primary Antibody Dilution : 1:1000
Secondary Antibody : Goat anti-rabbit-IRDye800
Secondry Antibody Dilution : 1:10,000
Submitted by: Ruben van Vugt, The Nijmegen Centre for Molecular Life Sciences (NCMLS)
Image 2
Rat
Species+tissue/cell type: Total rat cardiac lysate
1: 8 ug total cardiac lysate
2: 15 ug total cardiac lysate
3: 30 ug total cardiac lysate
4: 50 ug total cardiac lysatePrimary antibody dilution: 0.25 ug/ml
Image 3
Rat cardiac
Lanes:
Lane1: 8 ug total cardiac lysate
Lane2: 15 ug total cardiac lysate
Lane3: 30 ug total cardiac lysate
Lane4: 50 ug total cardiac lysate
Primary Antibody Dilution:
0.25 ug/ml
Secondary Antibody:

Secondary Antibody Dilution:

Gene Name:
GJA1
Submitted by:
Anonymous
Image 4
Human Placenta
WB Suggested Anti-GJA1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Placenta
 

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