Product Number |
ARP36525_P050 |
Product Page |
www.avivasysbio.com/dhx30-antibody-n-terminal-region-arp36525-p050.html |
Name |
DHX30 Antibody - N-terminal region (ARP36525_P050) |
Protein Size (# AA) |
361 amino acids |
Molecular Weight |
40 kDa |
NCBI Gene Id |
22907 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
DEAH (Asp-Glu-Ala-His) box polypeptide 30 |
Alias Symbols |
DDX30, RETCOR, NEDMIAL |
Peptide Sequence |
Synthetic peptide located within the following region: AESGMAPGGPGEGDGSLVNASRDLLKEFPQPKNLLNSVIGRALGISHAKD |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Description of Target |
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DHX30 is a member of this family.DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein has 97% sequence identity with the mouse HELG protein. Alternative splicing of this gene generates 3 transcript variants. |
Protein Interactions |
SUMO2; SUMO3; IVNS1ABP; STAU1; GRSF1; UBC; SUMO1; NEDD8; Fbxl16; WWOX; SUZ12; RNF2; EZH2; NEDD4; TARDBP; HNRNPD; EIF2AK2; SKIL; FN1; ESR1; NDUFA2; ILF3; GNAI2; RAB31; GRK5; CAND1; COPS5; CUL1; CUL3; CUL4B; CUL5; SIRT7; HNRNPA1; HDGF; DHX58; OAS3; Sgol2; Y |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-DHX30 (ARP36525_P050) antibody |
Blocking Peptide |
For anti-DHX30 (ARP36525_P050) antibody is Catalog # AAP36525 (Previous Catalog # AAPS07208) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human DHX30 |
Uniprot ID |
Q7L2E3-2 |
Protein Name |
Putative ATP-dependent RNA helicase DHX30 |
Sample Type Confirmation |
DHX30 is strongly supported by BioGPS gene expression data to be expressed in Jurkat |
Protein Accession # |
NP_619519 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_138614 |
Tested Species Reactivity |
Human |
Gene Symbol |
DHX30 |
Predicted Species Reactivity |
Human, Mouse, Cow, Dog, Pig, Rabbit |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 93%; Human: 100%; Mouse: 93%; Pig: 93%; Rabbit: 93% |
Image 1 | Human Adult Liver
| Rabbit Anti-DHX30 Antibody
Catalog Number: ARP36525_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver
Observed Staining: Cytoplasm in hepatocytes, moderate signal, wide tissue distribution
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 2.0 sec
Protocol located in Reviews and Data. |
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Image 2 | Human Jurkat
| WB Suggested Anti-DHX30 Antibody Titration: 0.2-1 ug/ml Positive Control: Jurkat cell lysateDHX30 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells |
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Image 3 | Human Lung Tissue
| Rabbit Anti-DHX30 Antibody Catalog Number: ARP36525_P050 Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue Observed Staining: Cytoplasmic in alveolar type I cells Primary Antibody Concentration: 1:100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
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