DHX30 Antibody - N-terminal region (ARP36525_P050)

Data Sheet
 
Product Number ARP36525_P050
Product Page www.avivasysbio.com/dhx30-antibody-n-terminal-region-arp36525-p050.html
Name DHX30 Antibody - N-terminal region (ARP36525_P050)
Protein Size (# AA) 361 amino acids
Molecular Weight 40 kDa
NCBI Gene Id 22907
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 30
Alias Symbols DDX30, RETCOR, NEDMIAL
Peptide Sequence Synthetic peptide located within the following region: AESGMAPGGPGEGDGSLVNASRDLLKEFPQPKNLLNSVIGRALGISHAKD
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Description of Target DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DHX30 is a member of this family.DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein has 97% sequence identity with the mouse HELG protein. Alternative splicing of this gene generates 3 transcript variants.
Protein Interactions SUMO2; SUMO3; IVNS1ABP; STAU1; GRSF1; UBC; SUMO1; NEDD8; Fbxl16; WWOX; SUZ12; RNF2; EZH2; NEDD4; TARDBP; HNRNPD; EIF2AK2; SKIL; FN1; ESR1; NDUFA2; ILF3; GNAI2; RAB31; GRK5; CAND1; COPS5; CUL1; CUL3; CUL4B; CUL5; SIRT7; HNRNPA1; HDGF; DHX58; OAS3; Sgol2; Y
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-DHX30 (ARP36525_P050) antibody
Blocking Peptide For anti-DHX30 (ARP36525_P050) antibody is Catalog # AAP36525 (Previous Catalog # AAPS07208)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human DHX30
Uniprot ID Q7L2E3-2
Protein Name Putative ATP-dependent RNA helicase DHX30
Sample Type Confirmation

DHX30 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

Protein Accession # NP_619519
Purification Affinity Purified
Nucleotide Accession # NM_138614
Tested Species Reactivity Human
Gene Symbol DHX30
Predicted Species Reactivity Human, Mouse, Cow, Dog, Pig, Rabbit
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 93%; Human: 100%; Mouse: 93%; Pig: 93%; Rabbit: 93%
Image 1
Human Adult Liver
Rabbit Anti-DHX30 Antibody
Catalog Number: ARP36525_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver
Observed Staining: Cytoplasm in hepatocytes, moderate signal, wide tissue distribution
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec
Protocol located in Reviews and Data.
Image 2
Human Jurkat
WB Suggested Anti-DHX30 Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat cell lysateDHX30 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Image 3
Human Lung Tissue
Rabbit Anti-DHX30 Antibody
Catalog Number: ARP36525_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
Observed Staining: Cytoplasmic in alveolar type I cells
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
 

AVIVA SYSTEMS BIOLOGY manufactures and sells quality antibody products covering genome wide proteins.

AVIVA SYSTEMS BIOLOGY
10211 Pacific Mesa Blvd, Ste 401, San Diego, CA 92121 USA | Tel: (858)552-6979 | info@avivasysbio.com