AKAP7 Antibody - N-terminal region (ARP36333_P050)

Data Sheet
 
Product Number ARP36333_P050
Product Page www.avivasysbio.com/akap7-antibody-n-terminal-region-arp36333-p050.html
Name AKAP7 Antibody - N-terminal region (ARP36333_P050)
Protein Size (# AA) 104 amino acids
Molecular Weight 11kDa
NCBI Gene Id 9465
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name A kinase (PRKA) anchor protein 7
Alias Symbols AKAP15, AKAP18
Peptide Sequence Synthetic peptide located within the following region: MGQLCCFPFSRDEGKISELESSSSAVLQRYSKDIPSWSSGEKNGGEPDDA
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Brown,R.L., et al., (2003) Biochem. Biophys. Res. Commun. 306 (2), 394-401
Description of Target AKAP7 is a member of the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular compartments. AKAPs have a common RII-binding domain, but contain different targeting motifs responsible for directing PKA to distinct intracellular locations. Three alternatively spliced transcript variants encoding different isoforms have been described. Additional variants exist, but their full-length natures have not been determined.
Protein Interactions CEP76; ROPN1; SPA17; PRKAR2B; PRKACB; PRKACA; MEPCE; USP4; PRKAR1A; PRKAR2A;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-AKAP7 (ARP36333_P050) antibody
Blocking Peptide For anti-AKAP7 (ARP36333_P050) antibody is Catalog # AAP36333 (Previous Catalog # AAPP07520)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human AKAP7
Uniprot ID O43687
Protein Name A-kinase anchor protein 7 isoforms alpha and beta
Protein Accession # NP_619539
Purification Affinity Purified
Nucleotide Accession # NM_138633
Tested Species Reactivity Human
Gene Symbol AKAP7
Predicted Species Reactivity Human
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Human: 100%
Image 1
Human Brain
WB Suggested Anti-AKAP7 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human brain
Image 2
heart
Rabbit Anti-AKAP7 Antibody
Catalog Number: ARP36333_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Membrane
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec
Protocol located in Reviews and Data.
 

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