ZIC2 Antibody - C-terminal region (ARP35821_P050)

• Product Number: ARP35821_P050
• Name: ZIC2 Antibody - C-terminal region (ARP35821_P050)
• Protein Size (# AA): 532 amino acids
• Molecular Weight: 55kDa
• NCBI Gene Id: 7546
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Zic family member 2
• Description:
  View product citations for ZIC2 Antibody - C-terminal region (ARP35821_P050) on CiteAb
• Alias Symbols: HPE5
• Peptide Sequence: Synthetic peptide located within the following region: EPQSSSNLSPAAAAAAAAAAAAAAAVSAVHRGGGSGSGGAGGGSGGGSGS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Brown,L., (2005) Hum. Mol. Genet. 14 (3), 411-420
• Description of Target: ZIC2 is a member of the ZIC family of C2H2-type zinc finger proteins. This protein functions as a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. Mutations in this gene cause holoprosencephaly type 5. Holoprosencephaly is the most common structural anomaly of the human brain. A polyhistidine tract polymorphism in this gene may be associated with increased risk of neural tube defects.This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This protein functions as a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. Mutations in this gene cause holoprosencephaly type 5. Holoprosencephaly is the most common structural anomaly of the human brain. A polyhistidine tract polymorphism in this gene may be associated with increased risk of neural tube defects. This gene is closely linked to a gene encoding zinc finger protein of the cerebellum 5, a related family member on chromosome 13.
• Protein Interactions: SUMO1; ELAVL1; GLI3; GLI2; GLI1; RNF180; UBC;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-ZIC2 (ARP35821_P050) antibody is Catalog # AAP35821 (Previous Catalog # AAPP23577)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ZIC2
• Uniprot ID: O95409
• Protein Name: Zinc finger protein ZIC 2
• Publications:

  Addiction of pancreatic cancer cells to zinc-finger transcription factor ZIC2. Oncotarget. 6, 28257-68 (2015). 26318045

  The long noncoding RNA lncZic2 drives the self-renewal of liver tumor-initiating cells via the protein kinase C substrates MARCKS and MARCKSL1. J Biol Chem. 293, 7982-7992 (2018). 29588366

  ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells. J. Clin. Invest. 125, 3795-808 (2015). 26426078

• Protein Accession #: NP_009060
• Purification: Affinity Purified
• Nucleotide Accession #: NM_007129
• Tested Species Reactivity: Human
• Gene Symbol: ZIC2
• Predicted Species Reactivity: Human, Mouse, Rat, Guinea Pig
• Application: IHC, WB, IP
• Predicted Homology Based on Immunogen Sequence: Guinea Pig: 87%; Human: 100%; Mouse: 85%; Rat: 92%
• Image 1: Human Skin
  
  Human Skin
• Image 2: Human HepG2
  
  WB Suggested Anti-ZIC2 Antibody Titration: 0.2-1 ug/ml
  Positive Control: HepG2 cell lysate
• Image 3: Human HT1080 Whole Cell
  
  Host: Rabbit
  Target Name: ZIC2
  Sample Tissue: Human HT1080 Whole Cell
  Antibody Dilution: 1ug/ml
• Image 4: HEK293 Whole Cell Lysate
  
  ZIC2 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with ARP35821_P050 with 1:200 dilution. Western blot was performed using ARP35821_P050 at 1/1000 dilution.
  Lane 1: Control IP in HEK293 Whole Cell Lysate.
  Lane 2: ZIC2 IP with ARP35821_P050 in HEK293 Whole Cell Lysate.
  Lane 3: Input of HEK293 Whole Cell Lysate.