| Product Number |
ARP35731_T100 |
| Product Page |
www.avivasysbio.com/ilf2-antibody-c-terminal-region-arp35731-t100.html |
| Name |
ILF2 Antibody - C-terminal region (ARP35731_T100) |
| Protein Size (# AA) |
390 amino acids |
| Molecular Weight |
43 kDa |
| NCBI Gene Id |
3608 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Interleukin enhancer binding factor 2, 45kDa |
| Alias Symbols |
NF45, PRO3063 |
| Peptide Sequence |
Synthetic peptide located within the following region: HGGFRKILGQEGDASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEEEE |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Shin,H.J., et al., (2002) Arch. Virol. 147 (3), 471-491 |
| Description of Target |
Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of the ILF2 gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. |
| Protein Interactions |
CCNDBP1; HUWE1; UBC; EEF1G; AURKA; SUMO2; SUMO3; STAU1; IVNS1ABP; RPA1; SMURF2; RPA3; RPA2; ERG; EZH2; BMI1; SUZ12; EED; rev; HNRNPU; FKBP3; DHX9; DDX1; ABCF1; C14orf166; RTCB; DIMT1; NELFB; EDC4; IGF2BP3; EIF2B2; EIF2B3; YBX3; RFC4; PTBP1; YBX1; NMT1; MR |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Enhanced Validation |
| Relative Expression (Western Blot) |
 |
|
| Datasheets/Manuals |
Printable datasheet for anti-ILF2 (ARP35731_T100) antibody |
| Blocking Peptide |
For anti-ILF2 (ARP35731_T100) antibody is Catalog # AAP35731 (Previous Catalog # AAPP06977) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human ILF2 |
| Uniprot ID |
Q12905 |
| Protein Name |
Interleukin enhancer-binding factor 2 |
| Publications |
Jiang, D., Zhou, Y., Moxley, R. A. & Jarrett, H. W. Purification and identification of positive regulators binding to a novel element in the c-Jun promoter. Biochemistry 47, 9318-34 (2008). 18690718
Yamauchi, T. et al. Sepantronium bromide (YM155) induces disruption of the ILF3/p54(nrb) complex, which is required for survivin expression. Biochem. Biophys. Res. Commun. 425, 711-6 (2012). 22842455 |
| Sample Type Confirmation |
ILF2 is supported by BioGPS gene expression data to be expressed in MCF7 |
| Protein Accession # |
NP_004506 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_004515 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
ILF2 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93% |
| Image 1 | Human kidney
 | Human kidney |
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| Image 2 | Human Heart
 | Rabbit Anti-ILF2 Antibody
Catalog Number: ARP35731
Paraffin Embedded Tissue: Human cardiac cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 3 |
 | 25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The protein may be modified by glycosylation and phosphorylation.
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