KCNN3 Antibody - C-terminal region (ARP35097_P050)

• Product Number: ARP35097_P050
• Name: KCNN3 Antibody - C-terminal region (ARP35097_P050)
• Protein Size (# AA): 736 amino acids
• Molecular Weight: 81kDa
• NCBI Gene Id: 3782
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3
• Alias Symbols: SK3, ZLS3, hSK3, SKCA3, KCa2.3
• Peptide Sequence: Synthetic peptide located within the following region: ITELNDRSEDLEKQIGSLESKLEHLTASFNSLPLLIADTLRQQQQQLLSA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Kolski-Andreaco,A., et al., (2004) J. Biol. Chem. 279 (8), 6893-6904
• Description of Target: Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. KCNN3, a member of the KCNN family of potassium channel genes, encodes a protein that is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits.
• Protein Interactions: UBC; RAB5A; DNM2; CAV1; APP; CALM1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-KCNN3 (ARP35097_P050) antibody is Catalog # AAP35097 (Previous Catalog # AAPP06328)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human KCNN3
• Uniprot ID: Q9UGI6
• Protein Name: Small conductance calcium-activated potassium channel protein 3
• Sample Type Confirmation:

  KCNN3 is supported by BioGPS gene expression data to be expressed in Daudi

• Protein Accession #: NP_002240
• Purification: Affinity Purified
• Nucleotide Accession #: NM_002249
• Tested Species Reactivity: Human, Mouse, Monkey
• Gene Symbol: KCNN3
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human 293T Whole Cell
  
  Host: Rabbit
  Target Name: KCNN3
  Sample Tissue: Human 293T Whole Cell
  Antibody Dilution: 1ug/ml
• Image 2: Mouse kidney, brain
  
  KCNN3 antibody - C-terminal region (ARP35097_P050) validated by WB using Mouse kidney, Whole brain lysate at 2 ug/ml.
• Image 3: heart
  
  Rabbit Anti-KCNN3 Antibody
  Catalog Number: ARP35097_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
  Observed Staining: Membrane
  Primary Antibody Concentration: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy2/3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 – 2.0 sec
  Protocol located in Reviews and Data.
• Image 4: Rhesus macaque spinal cord
  
  Sample Type:
  Rhesus macaque spinal cord
  Primary Antibody Dilution:
  1:300
  Secondary Antibody:
  Donkey anti Rabbit 488
  Secondary Antibody Dilution:
  1:500
  Color/Signal Descriptions:
  Green: KCNN3
  Gene Name:
  KCNN3
  Submitted by:
  Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706
• Image 5: Human Daudi
  
  WB Suggested Anti-KCNN3 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: Daudi cell lysateKCNN3 is supported by BioGPS gene expression data to be expressed in Daudi
• Image 6: Human THP-1 Whole Cell
  
  Host: Rabbit
  Target Name: KCNN3
  Sample Tissue: Human THP-1 Whole Cell
  Antibody Dilution: 1ug/ml
• Image 7: Human MCF7 Whole Cell
  
  Host: Rabbit
  Target Name: KCNN3
  Sample Tissue: Human MCF7 Whole Cell
  Antibody Dilution: 0.5ug/ml