THRA Antibody - middle region (ARP34780_P050)

• Product Number: ARP34780_P050
• Name: THRA Antibody - middle region (ARP34780_P050)
• Protein Size (# AA): 490 amino acids
• Molecular Weight: 55kDa
• NCBI Gene Id: 7067
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Thyroid hormone receptor, alpha
• Alias Symbols: AR7, EAR7, ERBA, CHNG6, ERBA1, NR1A1, THRA1, THRA2, ERB-T-1, c-ERBA-1
• Peptide Sequence: Synthetic peptide located within the following region: DQIILLKGCCMEIMSLRAAVRYDPESDTLTLSGEMAVKREQLKNGGLGVV
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: The THRA gene encodes a protein that is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone.
• Protein Interactions: L3MBTL3; AMOTL2; RANBP9; NCOR1; PTGES3; THRA; RXRA; FOXJ1; CLIC1; C1D; NCOR2; BUB3; tat; GTF2B; ARID5A; SUMO1; SUMO3; MED1; NRIP1; MED21; CDK7; MED25; RGN; CUL1; SEPP1; TBL1XR1; HDAC3; SMC1A; XRCC5; TBL1X; PRKDC; LIG3; HSPA4; GPS2; XRCC6; PARP1; EMD; GRIP
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-THRA (ARP34780_P050) antibody is Catalog # AAP34780 (Previous Catalog # AAPP05974)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human THRA
• Uniprot ID: P10827
• Protein Name: Thyroid hormone receptor alpha
• Publications:

  Kiss-Toth, E., Harlock, E., Lath, D., Quertermous, T. & Wilkinson, J. M. A TNF variant that associates with susceptibility to musculoskeletal disease modulates thyroid hormone receptor binding to control promoter activation. PLoS One 8, e76034 (2013). 24069456

• Sample Type Confirmation:

  There is BioGPS gene expression data showing that THRA is expressed in Jurkat

• Protein Accession #: NP_003241
• Purification: Affinity Purified
• Nucleotide Accession #: NM_003250
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: THRA
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
• Image 1: Human Jurkat
  
  WB Suggested Anti-THRA Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: Jurkat cell lysateThere is BioGPS gene expression data showing that THRA is expressed in Jurkat
• Image 2: Mouse Spleen
  
  Host: Mouse
  Target Name: THRA
  Sample Tissue: Mouse Spleen
  Antibody Dilution: 1ug/ml
• Image 3: Human Ovary, Human stomach
  
  Host: Rabbit
  Target: THRA
  Positive control (+): Human Ovary (OV)
  Negative control (-): Human stomach (ST)
  Antibody concentration: 0.5ug/ml
• Image 4: Mouse Heart
  
  Host: Mouse
  Target Name: THRA
  Sample Tissue: Mouse Heart
  Antibody Dilution: 1ug/ml
• Image 5: Human heart
  
  WB Suggested Anti-THRA antibody Titration: 1 ug/mL
  Sample Type: Human heart