KEAP1 Antibody - N-terminal region (ARP34727_P050)

Data Sheet
 
Product Number ARP34727_P050
Product Page www.avivasysbio.com/keap1-antibody-n-terminal-region-arp34727-p050.html
Name KEAP1 Antibody - N-terminal region (ARP34727_P050)
Protein Size (# AA) 624 amino acids
Molecular Weight 70kDa
NCBI Gene Id 9817
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Kelch-like ECH-associated protein 1
Alias Symbols INrf2, KLHL19
Peptide Sequence Synthetic peptide located within the following region: SQCPEGAGDAVMYASTECKAEVTPSQHGNRTFSYTLEDHTKQAFGIMNEL
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Padmanabhan,B., (2006) Mol. Cell 21 (5), 689-700
Description of Target KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Protein Interactions OXR1; ZSCAN32; LSM3; KLHL3; FBXW11; MAPKBP1; NUDT4; DPP3; RBX1; ATG5; SQSTM1; PTMA; PRKAG1; NFE2L2; MAD2L1; GYPA; ETF1; BCL2; CUL3; RECK; UBC; PGAM5; FAM117B; WDR83; CCNB1IP1; HSP90AA1; XPO1; MCMBP; KEAP1; SLK; MCM3; IKBKB; AMER1; GPRASP2; PRKCE; NFE2L1;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-KEAP1 (ARP34727_P050) antibody
Additional Information IHC Information: Paraffin embedded prostate tissue, tested with an antibody dilution of 5 ug/ml.
Blocking Peptide For anti-KEAP1 (ARP34727_P050) antibody is Catalog # AAP34727 (Previous Catalog # AAPP05921)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human KEAP1
Uniprot ID Q14145
Protein Name Kelch-like ECH-associated protein 1
Protein Accession # NP_987096
Purification Affinity Purified
Nucleotide Accession # NM_203500
Tested Species Reactivity Human
Gene Symbol KEAP1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 77%
Image 1
Transfected 293T
WB Suggested Anti-KEAP1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Transfected 293T
Image 2
Human Kidney
Kidney
Image 3
Human Lymph Node
Rabbit Anti-KEAP1 Antibody
Catalog Number: ARP34727_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lymph Node Tissue
Observed Staining: Cytoplasm, Nucleus
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
 

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