Product Number |
ARP34727_P050 |
Product Page |
www.avivasysbio.com/keap1-antibody-n-terminal-region-arp34727-p050.html |
Name |
KEAP1 Antibody - N-terminal region (ARP34727_P050) |
Protein Size (# AA) |
624 amino acids |
Molecular Weight |
70kDa |
NCBI Gene Id |
9817 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Kelch-like ECH-associated protein 1 |
Alias Symbols |
INrf2, KLHL19 |
Peptide Sequence |
Synthetic peptide located within the following region: SQCPEGAGDAVMYASTECKAEVTPSQHGNRTFSYTLEDHTKQAFGIMNEL |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Padmanabhan,B., (2006) Mol. Cell 21 (5), 689-700 |
Description of Target |
KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. |
Protein Interactions |
OXR1; ZSCAN32; LSM3; KLHL3; FBXW11; MAPKBP1; NUDT4; DPP3; RBX1; ATG5; SQSTM1; PTMA; PRKAG1; NFE2L2; MAD2L1; GYPA; ETF1; BCL2; CUL3; RECK; UBC; PGAM5; FAM117B; WDR83; CCNB1IP1; HSP90AA1; XPO1; MCMBP; KEAP1; SLK; MCM3; IKBKB; AMER1; GPRASP2; PRKCE; NFE2L1; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-KEAP1 (ARP34727_P050) antibody |
Additional Information |
IHC Information: Paraffin embedded prostate tissue, tested with an antibody dilution of 5 ug/ml. |
Blocking Peptide |
For anti-KEAP1 (ARP34727_P050) antibody is Catalog # AAP34727 (Previous Catalog # AAPP05921) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human KEAP1 |
Uniprot ID |
Q14145 |
Protein Name |
Kelch-like ECH-associated protein 1 |
Protein Accession # |
NP_987096 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_203500 |
Tested Species Reactivity |
Human |
Gene Symbol |
KEAP1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 77% |
Image 1 | Transfected 293T
| WB Suggested Anti-KEAP1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: Transfected 293T |
|
Image 2 | Human Kidney
| Kidney |
|
Image 3 | Human Lymph Node
| Rabbit Anti-KEAP1 Antibody Catalog Number: ARP34727_P050 Formalin Fixed Paraffin Embedded Tissue: Human Lymph Node Tissue Observed Staining: Cytoplasm, Nucleus Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
|