| Product Number |
ARP34680_T100 |
| Product Page |
www.avivasysbio.com/mid1-antibody-c-terminal-region-arp34680-t100.html |
| Name |
MID1 Antibody - C-terminal region (ARP34680_T100) |
| Protein Size (# AA) |
667 amino acids |
| Molecular Weight |
75kDa |
| NCBI Gene Id |
4281 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Midline 1 (Opitz/BBB syndrome) |
| Alias Symbols |
OS, FXY, OSX, OGS1, XPRF, BBBG1, GBBB1, MIDIN, RNF59, ZNFXY, TRIM18 |
| Peptide Sequence |
Synthetic peptide located within the following region: AINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSK |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Winter,J., et al., (2004) Hum. Genet. 114 (6), 541-552 |
| Description of Target |
MID1 is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Several different transcript variants are generated by alternate splicing; however, the full length nature of two variants has not been determined. |
| Protein Interactions |
STK36; MID2; UBE2E3; DYRK4; UBE2E2; UBE2E1; UBE2D3; UBE2D2; UBE2D1; PKN1; PPP2R4; PPP2CA; MID1; MEOX1; FKBP1A; EHHADH; BYSL; TCEANC; UBTD1; OTUB2; GMCL1; HTT; IGBP1; PNKP; MID1IP1; GNB2L1; RPS8; RPS3; NPM1; HSP90AA1; EEF1A1; ANXA2; UBC; UBE2D4; UBE2N; ELA |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-MID1 (ARP34680_T100) antibody |
| Blocking Peptide |
For anti-MID1 (ARP34680_T100) antibody is Catalog # AAP34680 (Previous Catalog # AAPP05870) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human MID1 |
| Uniprot ID |
O15344 |
| Protein Name |
Midline-1 |
| Protein Accession # |
NP_000372 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_000381 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
MID1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-MID1 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate |
|
