NFKBIB Antibody - middle region (ARP34381_P050)

• Product Number: ARP34381_P050
• Name: NFKBIB Antibody - middle region (ARP34381_P050)
• Protein Size (# AA): 356 amino acids
• Molecular Weight: 38kDa
• NCBI Gene Id: 4793
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
• Alias Symbols: IKBB, TRIP9
• Peptide Sequence: Synthetic peptide located within the following region: PILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Kerppola,T.K. (er) Ann. Rheum. Dis. (2008) In press
• Description of Target: Inhibition of NFkappa-B activity by the hepatitis C virus core protein might be related to its physical interaction with and interrupted nuclear localization of IKKbeta. Increased nuclear factor-kappaB (NF-kB) activity in the amnion during labor is associated with an increase in the expression of NF-kBp65 and of the NF-kB binding proteins IkBa, IkBb-1 and IkBb-2. IkappaBbeta may be a novel target for transcription factors of the HMG-box SRY/Sox family and imply a potential role for NF-kappaB/IkappaBbeta in spermatogenesis. NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-766 BM449613.1 1-766 767-1189 BU634404.1 18-440 c 1190-1198 AI684894.1 1-9 c
• Protein Interactions: VPS52; UBC; BAG3; TSPAN1; GIT2; RELA; REL; CSNK2A1; CDC25A; BAK1; APP; ZBTB7A; DNAJA3; IKBKB; CHUK; BRAP; IKBKG; TRE17; NFKBIA; NCOR2; PPARG; ESR2; POLR1D; POLR1E; POLR1A; POLR1C; POLR2H; NKIRAS2; POLR1B; NKIRAS1; LRPPRC; RASAL2; MTIF2; BTRC; CUL1; SKP1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-NFKBIB (ARP34381_P050) antibody is Catalog # AAP34381 (Previous Catalog # AAPY00223)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFKBIB
• Uniprot ID: Q15653
• Protein Name: NF-kappa-B inhibitor beta
• Sample Type Confirmation:

  NFKBIB is strongly supported by BioGPS gene expression data to be expressed in 721_B

• Protein Accession #: NP_002494
• Purification: Affinity Purified
• Nucleotide Accession #: NM_002503
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: NFKBIB
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 91%; Rat: 100%
• Image 1: Human 721_B
  
  WB Suggested Anti-NFKBIB Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: 721_B cell lysateNFKBIB is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
• Image 2: Human Testis
  
  Rabbit Anti-NFKBIB Antibody
  Catalog Number: ARP34381_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Testis Tissue
  Observed Staining: Nucleus, Cytoplasm
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: N/A
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 3: Mouse Testis
  
  Host: Rabbit
  Target Name: NFKBIB
  Sample Tissue: Mouse Testis
  Antibody Dilution: 1ug/ml
• Image 4: Mouse Testis
  
  Host: Mouse
  Target Name: NFKBIB
  Sample Tissue: Mouse Testis
  Antibody Dilution: 1ug/ml