| Product Number |
ARP34377_P050 |
| Product Page |
www.avivasysbio.com/eno1-antibody-middle-region-arp34377-p050.html |
| Name |
ENO1 Antibody - middle region (ARP34377_P050) |
| Protein Size (# AA) |
434 amino acids |
| Molecular Weight |
47kDa |
| NCBI Gene Id |
2023 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Enolase 1, (alpha) |
| Alias Symbols |
NNE, PPH, MPB1, ENO1L1, HEL-S-17 |
| Peptide Sequence |
Synthetic peptide located within the following region: VAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDP |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Lee,K.H., et al., (2003) Arthritis Rheum. 48 (7), 2025-2035 |
| Description of Target |
ENO1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. |
| Protein Interactions |
UBC; FUS; HUWE1; ISG15; SUMO2; SUMO3; IVNS1ABP; STAU1; MDM2; WWOX; EED; FBXO6; UPF2; YWHAQ; UBD; TERT; SRC; RAD52; NPM1; BHLHE40; VCAM1; HCVgp1; ITGA4; FN1; ATF2; SERPING1; AMBP; LIG4; BRCA1; MDC1; PGK1; ITPA; GAPDH; FYN; FLNC; ENO3; Htt; TPI1; PPIA; AKT1 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-ENO1 (ARP34377_P050) antibody |
| Blocking Peptide |
For anti-ENO1 (ARP34377_P050) antibody is Catalog # AAP34377 (Previous Catalog # AAPY00219) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human ENO1 |
| Uniprot ID |
Q53HR3 |
| Protein Name |
Alpha-enolase |
| Publications |
Boraldi, F. et al. Hypoxia influences the cellular cross-talk of human dermal fibroblasts. A proteomic approach. Biochim. Biophys. Acta 1774, 1402-13 (2007). 17904921
Braceland, M. et al. Serum enolase: a non-destructive biomarker of white skeletal myopathy during pancreas disease (PD) in Atlantic salmon Salmo salar L. J. Fish Dis. doi:10.1111/jfd.12296 (2014). 25168106
Deng, M. Y. et al. Frontal-subcortical protein expression following prenatal exposure to maternal inflammation. PLoS One 6, e16638 (2011). 21347362
Kitahashi, T., Yoshimoto, M. & Imai, T. Novel immunohistochemical marker, integrin a(V)beta(3), for BOP-induced early lesions in hamster pancreatic ductal carcinogenesis. Oncol. Lett. 2, 229-234 (2011). 22866069
Sørensen, B. S. et al. Proteins upregulated by mild and severe hypoxia in squamous cell carcinomas in vitro identified by proteomics. Radiother. Oncol. 92, 443-9 (2009). 19541378 |
| Protein Accession # |
NP_001419 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001428 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
ENO1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 79%; Dog: 93%; Guinea Pig: 93%; Horse: 79%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 93% |
| Image 1 | Human Placenta
 | Rabbit Anti-ENO1 Antibody
Catalog Number: ARP34377
Paraffin Embedded Tissue: Human placenta cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 2 | Human kidney
 | Human kidney |
|
| Image 3 | Human Small Intestine
 | WB Suggested Anti-ENO1 Antibody Titration: 0.03ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Small Intestine |
|
| Image 4 | Human heart
 | WB Suggested Anti-ENO1 antibody Titration: 1 ug/mL
Sample Type: Human heart |
|
| Image 5 | Human liver
 | WB Suggested Anti-ENO1 antibody Titration: 1 ug/mL
Sample Type: Human liver |
|
| Image 6 | heart
 | Rabbit Anti-ENO1 Antibody
Catalog Number: ARP34377_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Cytoplasmic,Membrane
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 2.0 sec
Protocol located in Reviews and Data. |
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