| Product Number |
ARP34376_T100 |
| Product Page |
www.avivasysbio.com/eno1-antibody-c-terminal-region-arp34376-t100.html |
| Name |
ENO1 Antibody - C-terminal region (ARP34376_T100) |
| Protein Size (# AA) |
434 amino acids |
| Molecular Weight |
47kDa |
| NCBI Gene Id |
2023 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Enolase 1, (alpha) |
| Alias Symbols |
NNE, PPH, MPB1, ENO1L1, HEL-S-17 |
| Peptide Sequence |
Synthetic peptide located within the following region: VVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Ghosh,A.K., et al., (2005) J. Biol. Chem. 280 (14), 14325-14330 |
| Description of Target |
ENO1 is one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. |
| Protein Interactions |
UBC; FUS; HUWE1; ISG15; SUMO2; SUMO3; IVNS1ABP; STAU1; MDM2; WWOX; EED; FBXO6; UPF2; YWHAQ; UBD; TERT; SRC; RAD52; NPM1; BHLHE40; VCAM1; HCVgp1; ITGA4; FN1; ATF2; SERPING1; AMBP; LIG4; BRCA1; MDC1; PGK1; ITPA; GAPDH; FYN; FLNC; ENO3; Htt; TPI1; PPIA; AKT1 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Enhanced Validation |
| Relative Expression (Western Blot) |
 |
|
| Datasheets/Manuals |
Printable datasheet for anti-ENO1 (ARP34376_T100) antibody |
| Blocking Peptide |
For anti-ENO1 (ARP34376_T100) antibody is Catalog # AAP34376 (Previous Catalog # AAPY00218) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human ENO1 |
| Uniprot ID |
Q53HR3 |
| Protein Name |
Alpha-enolase |
| Publications |
Rambaruth, N. D. S., Greenwell, P. & Dwek, M. V. The lectin Helix pomatia agglutinin recognizes O-GlcNAc containing glycoproteins in human breast cancer. Glycobiology 22, 839-48 (2012). 22322011 |
| Sample Type Confirmation |
ENO1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat |
| Protein Accession # |
NP_001419 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_001428 |
| Tested Species Reactivity |
Human, Rat |
| Gene Symbol |
ENO1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Yeast, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%; Sheep: 93%; Yeast: 79%; Zebrafish: 79% |
| Image 1 | Rat Skeletal Muscle
 | Host: Rat
Target Name: ENO1
Sample Tissue: Rat Skeletal Muscle
Antibody Dilution: 1ug/ml |
|
| Image 2 | Human Lung
 | Rabbit Anti-ENO1 Antibody
Catalog Number: ARP34376
Paraffin Embedded Tissue: Human alveolar cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 3 | Human Jurkat
 | WB Suggested Anti-ENO1 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysateENO1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells |
|
