CCT4 Antibody - C-terminal region (ARP34271_T100)

Data Sheet
 
Product Number ARP34271_T100
Product Page www.avivasysbio.com/cct4-antibody-c-terminal-region-arp34271-t100.html
Name CCT4 Antibody - C-terminal region (ARP34271_T100)
Protein Size (# AA) 539 amino acids
Molecular Weight 58kDa
Subunit delta
NCBI Gene Id 10575
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Chaperonin containing TCP1, subunit 4 (delta)
Alias Symbols SRB, Cctd, CCT-DELTA
Peptide Sequence Synthetic peptide located within the following region: AFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISN
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Kimura,K., et al., (2006) Genome Res. 16 (1), 55-65
Description of Target CCT4 is a subunit of a cytosolic hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. This protein is a member of the chaperonin family, which includes Escherichia coli GroEL, the mitochondrial heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein and the archaebacterial protein TF55.
Protein Interactions HUWE1; TP53; FUS; CDC20; NEDD1; TUBG1; LGALS3BP; UBC; STAU1; MDM2; RFWD2; VRK2; PRPF40A; CCT7; USP9X; VARS; CCT3; CCT6A; FBXO6; UBD; FBXW4; CDK9; CDK5; CDK2; CD81; IGSF8; HDAC11; HDAC3; RPS6KA1; ILK; ICAM1; PAN2; VCAM1; PPP5C; PEX14; NOS2; US3; FAM86B2; C
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-CCT4 (ARP34271_T100) antibody
Blocking Peptide For anti-CCT4 (ARP34271_T100) antibody is Catalog # AAP34271
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human CCT4
Uniprot ID Q53QP9
Protein Name T-complex protein 1 subunit delta
Sample Type Confirmation

CCT4 is supported by BioGPS gene expression data to be expressed in HEK293T

Protein Accession # NP_006421
Purification Protein A purified
Nucleotide Accession # NM_006430
Tested Species Reactivity Human, Rat
Gene Symbol CCT4
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 93%; Goat: 79%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 100%; Yeast: 100%; Zebrafish: 93%
Image 1
Human HEK293T
Lanes:
Lane1: Negative control (anti-FLAG antibody)
Lane2: Immunoprecipitation with CCT4 (C-term)
Lane3: 10 ug lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-rabbit
Secondary Antibody Dilution:
1:10000
Gene Name:
CCT4
Submitted by:
Amy Gray, Department of Chemistry and Biochemistry Brigham Young University
CCT4 is supported by BioGPS gene expression data to be expressed in HEK293T
Image 2
Human HepG2
WB Suggested Anti-CCT4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysateCCT4 is supported by BioGPS gene expression data to be expressed in HepG2
Image 3
Human 293T
CCT4 antibody - C-terminal region (ARP34271_T100) validated by WB using 293T cells lysateCCT4 is supported by BioGPS gene expression data to be expressed in HEK293T
Image 4
Rat Hippocampal Neurons
Sample Type : Rat Hippocampal Neurons - 14DIV Primary Antibody Dilution : 1:200 Secondary Antibody : Anti-rabbit-Cy3 Secondary Antibody Dilution : 1:500 Color/Signal Descriptions : White: CCT4 Gene Name : CCT4 Submitted by : Dan Fowler - University of Oregon, Institute of Neuroscience
 

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