Product Number |
ARP34271_T100 |
Product Page |
www.avivasysbio.com/cct4-antibody-c-terminal-region-arp34271-t100.html |
Name |
CCT4 Antibody - C-terminal region (ARP34271_T100) |
Protein Size (# AA) |
539 amino acids |
Molecular Weight |
58kDa |
Subunit |
delta |
NCBI Gene Id |
10575 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Chaperonin containing TCP1, subunit 4 (delta) |
Alias Symbols |
SRB, Cctd, CCT-DELTA |
Peptide Sequence |
Synthetic peptide located within the following region: AFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISN |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Kimura,K., et al., (2006) Genome Res. 16 (1), 55-65 |
Description of Target |
CCT4 is a subunit of a cytosolic hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. This protein is a member of the chaperonin family, which includes Escherichia coli GroEL, the mitochondrial heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein and the archaebacterial protein TF55. |
Protein Interactions |
HUWE1; TP53; FUS; CDC20; NEDD1; TUBG1; LGALS3BP; UBC; STAU1; MDM2; RFWD2; VRK2; PRPF40A; CCT7; USP9X; VARS; CCT3; CCT6A; FBXO6; UBD; FBXW4; CDK9; CDK5; CDK2; CD81; IGSF8; HDAC11; HDAC3; RPS6KA1; ILK; ICAM1; PAN2; VCAM1; PPP5C; PEX14; NOS2; US3; FAM86B2; C |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-CCT4 (ARP34271_T100) antibody |
Blocking Peptide |
For anti-CCT4 (ARP34271_T100) antibody is Catalog # AAP34271 |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human CCT4 |
Uniprot ID |
Q53QP9 |
Protein Name |
T-complex protein 1 subunit delta |
Sample Type Confirmation |
CCT4 is supported by BioGPS gene expression data to be expressed in HEK293T |
Protein Accession # |
NP_006421 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_006430 |
Tested Species Reactivity |
Human, Rat |
Gene Symbol |
CCT4 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 93%; Goat: 79%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 100%; Yeast: 100%; Zebrafish: 93% |
Image 1 | Human HEK293T
| Lanes: Lane1: Negative control (anti-FLAG antibody) Lane2: Immunoprecipitation with CCT4 (C-term) Lane3: 10 ug lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Goat anti-rabbit Secondary Antibody Dilution: 1:10000 Gene Name: CCT4 Submitted by: Amy Gray, Department of Chemistry and Biochemistry Brigham Young University CCT4 is supported by BioGPS gene expression data to be expressed in HEK293T |
|
Image 2 | Human HepG2
| WB Suggested Anti-CCT4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysateCCT4 is supported by BioGPS gene expression data to be expressed in HepG2 |
|
Image 3 | Human 293T
| CCT4 antibody - C-terminal region (ARP34271_T100) validated by WB using 293T cells lysateCCT4 is supported by BioGPS gene expression data to be expressed in HEK293T |
|
Image 4 | Rat Hippocampal Neurons
| Sample Type :
Rat Hippocampal Neurons - 14DIV
Primary Antibody Dilution :
1:200
Secondary Antibody :
Anti-rabbit-Cy3
Secondary Antibody Dilution :
1:500
Color/Signal Descriptions :
White: CCT4
Gene Name :
CCT4
Submitted by :
Dan Fowler - University of Oregon, Institute of Neuroscience
|
|