Product Number |
ARP34192_P050 |
Product Page |
www.avivasysbio.com/crsp6-antibody-n-terminal-region-arp34192-p050.html |
Name |
CRSP6 Antibody - N-terminal region (ARP34192_P050) |
Protein Size (# AA) |
651 amino acids |
Molecular Weight |
73kDa |
Subunit |
17 |
NCBI Gene Id |
9440 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Mediator complex subunit 17 |
Description |
|
Alias Symbols |
SRB4, CRSP6, CRSP77, DRIP80, TRAP80 |
Peptide Sequence |
Synthetic peptide located within the following region: AAQILLKGAERLTKSVTENQENKLQRDFNSELLRLRQHWKLRKVGDKILG |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Wang,Q., et al., (2002) J. Biol. Chem. 277 (45), 42852-42858 |
Description of Target |
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by CRSP6 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. |
Protein Interactions |
UBC; BRCA1; CDK19; CDK8; MED26; POLR2C; ESR2; MED19; FBXW7; EPAS1; TFIP11; MED24; MED21; MED14; MED11; MED28; WDR33; CPSF2; UBD; BCL6; SMARCA4; SUZ12; MED10; CTDP1; SREBF1; MED25; MED15; TRA; MED1; OBFC1; MED18; ZC3H13; MED12; QKI; TRIP4; TADA2A; SUPT7L; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Enhanced Validation |
|
Datasheets/Manuals |
Printable datasheet for anti-MED17 (ARP34192_P050) antibody |
Blocking Peptide |
For anti-MED17 (ARP34192_P050) antibody is Catalog # AAP34192 (Previous Catalog # AAPP05507) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human CRSP6 |
Uniprot ID |
Q9NVC6 |
Protein Name |
Mediator of RNA polymerase II transcription subunit 17 |
Publications |
DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes. Nat Commun. 5, 4263 (2014). 24968874
Hepatic TRAP80 selectively regulates lipogenic activity of liver X receptor. J Clin Invest. 125, 183-93 (2015). 25437875 |
Sample Type Confirmation |
MED17 is supported by BioGPS gene expression data to be expressed in HepG2 |
Protein Accession # |
NP_004259 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_004268 |
Tested Species Reactivity |
Human |
Gene Symbol |
MED17 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
IHC, WB, CHIP |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 100% |
Image 1 | Human Brain
| Human Brain |
|
Image 2 | HCT116
| Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site. |
|
Image 3 | Human HepG2
| WB Suggested Anti-CRSP6 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:12500 Positive Control: HepG2 cell lysateMED17 is supported by BioGPS gene expression data to be expressed in HepG2 |
|
Image 4 | Human Brain
| Rabbit Anti-MED17 Antibody Catalog Number: ARP34192 Paraffin Embedded Tissue: Human neural cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X |
|
Image 5 | Human HepG2 Whole Cell
| Host: Rabbit Target Name: MED17 Sample Tissue: Human HepG2 Whole Cell Antibody Dilution: 1ug/ml |
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