| Product Number |
ARP34163_T100 |
| Product Page |
www.avivasysbio.com/siah1-antibody-n-terminal-region-arp34163-t100.html |
| Name |
SIAH1 Antibody - N-terminal region (ARP34163_T100) |
| Protein Size (# AA) |
313 amino acids |
| Molecular Weight |
35kDa |
| NCBI Gene Id |
6477 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Siah E3 ubiquitin protein ligase 1 |
| Alias Symbols |
SIAH1A |
| Peptide Sequence |
Synthetic peptide located within the following region: FTCLPAARTRKRKEMSRQTATALPTGTSKCPPSQRVPALTGTTASNNDLA |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Avraham,E., et al., (2005) J. Biol. Chem. 280 (52), 42877-42886 |
| Description of Target |
SIAH1 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. |
| Protein Interactions |
MX1; MAB21L1; KIFC3; KCNJ10; INHA; UBE2K; FLI1; EEF1D; PHC2; PHC1; CDC34; CAPG; BCL6; TRIM23; AQP1; PFKM; ZCCHC13; MTIF3; UPP2; MOB3C; SPATA4; PRR20A; HIST4H4; EXOC3-AS1; PTPMT1; RPS19BP1; SPATC1L; KATNAL1; TRIM7; GDPD5; ZBP1; KIAA1683; ARMC9; KIAA0319L; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-SIAH1 (ARP34163_T100) antibody |
| Blocking Peptide |
For anti-SIAH1 (ARP34163_T100) antibody is Catalog # AAP34163 (Previous Catalog # AAPP05425) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human SIAH1 |
| Uniprot ID |
Q8IUQ4-2 |
| Protein Name |
E3 ubiquitin-protein ligase SIAH1 |
| Sample Type Confirmation |
SIAH1 is strongly supported by BioGPS gene expression data to be expressed in HEK293T |
| Protein Accession # |
NP_001006611 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_001006610 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
SIAH1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86% |
| Image 1 | Mouse Liver
 | Host: Mouse
Target Name: SIAH1A
Sample Tissue: Mouse Liver
Antibody Dilution: 1ug/ml |
|
| Image 2 | Human Jurkat
 | WB Suggested Anti-SIAH1 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate |
|
| Image 3 | Human Heart
 | Rabbit Anti-SIAH1 antibody
Catalog Number: ARP34163_T100
Paraffin Embedded Tissue: Human Heart cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X |
|
| Image 4 | Human HEK293T
 | Researcher: Peter Brand & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University JenaApplication: Western blottingSpecies+tissue/cell type: Human embryonic kidney 293THow many ugs of tissue/cell lysate run on the gel:1: 50 ug human HEK-293T cell lysatePrimary antibody dilution: 1:1000Secondary antibody: Anti-rabbit-IgGSecondary antibody dilution: 1:5000 - See more at: http://www.avivasysbio.com/anti-siah1-antibody-n-terminal-region-arp34163-t100.html#sthash.GIS7dILc.dpufSIAH1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells |
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