NR1H4 Antibody - middle region (ARP33672_P050)

• Product Number: ARP33672_P050
• Name: NR1H4 Antibody - middle region (ARP33672_P050)
• Protein Size (# AA): 472 amino acids
• Molecular Weight: 54kDa
• NCBI Gene Id: 9971
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Nuclear receptor subfamily 1, group H, member 4
• Alias Symbols: BAR, FXR, HRR1, HRR-1, PFIC5, RIP14
• Peptide Sequence: Synthetic peptide located within the following region: AECLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKSCREKT
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Pineda et al., (2004) J. Biol. Chem. 279 (35), 36184-36191
• Description of Target: The NR1H4 gene encodes a protein that has a central role in lipid homeostasis and also regulates carbohydrate metabolism.
• Protein Interactions: SMARCD3; HDAC6; SUMO1; NCOR2; ESR1; RRM2; GPS2; RXRG; GAPDH; RXRA; EP300; SIRT1; SRC; NCOA6; CARM1; HIST1H4A; HIST1H3A; PRMT1; NCOR1; XRCC5; PRKDC; XRCC6; NCOA1; CASP8; SMARCD1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-NR1H4 (ARP33672_P050) antibody is Catalog # AAP33672 (Previous Catalog # AAPP04734)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NR1H4
• Uniprot ID: Q96RI1-2
• Protein Name: Bile acid receptor
• Protein Accession #: NP_005114
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005123
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: NR1H4
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%
• Image 1: Human kidney
  
  Human kidney
• Image 2: Human 786-0
  
  Host: Rabbit
  Target Name: NR1H4
  Sample Tissue: Human 786-0
  Antibody Dilution: 1.0ug/ml
• Image 3: Human kidney, Human lung
  
  Host: Rabbit
  Target: NR1H4
  Positive control (+): Human kidney (KI)
  Negative control (-): Human lung (LU)
  Antibody concentration: 1ug/ml
• Image 4: mouse glomerular endothelial
  
  WB Suggested Anti-NR1H4 Antibody
  Positive Control: Lane 1: 50ug mouse glomerular endothelial lysate
  Primary Antibody Dilution : 1:1000
  Secondary Antibody : Anti-rabbit-HRP
  Secondry Antibody Dilution : 1:5000
  Submitted by: Xiaoxin Wang, UC Denver
• Image 5:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.