TBX1 Antibody - C-terminal region (ARP33399_T100)

• Product Number: ARP33399_T100
• Name: TBX1 Antibody - C-terminal region (ARP33399_T100)
• Protein Size (# AA): 398 amino acids
• Molecular Weight: 43 kDa
• NCBI Gene Id: 6899
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: T-box 1
• Alias Symbols: DGS, TGA, VCF, CAFS, CTHM, DGCR, DORV, VCFS, TBX1C, CATCH22
• Peptide Sequence: Synthetic peptide located within the following region: PNPELRLEAPGASEPLHHHPYKYPAAAYDHYLGAKSRPAPYPLPGLRGHG
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Yamagishi,H., et al., (2003) Genes Dev. 17 (2), 269-281
• Description of Target: TBX1 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. TBX1 product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where TBX1 has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Protein Interactions: TERF2; TERF1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-TBX1 (ARP33399_T100) antibody is Catalog # AAP33399 (Previous Catalog # AAPP04445)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TBX1
• Uniprot ID: O43435-3
• Protein Name: T-box transcription factor TBX1
• Protein Accession #: NP_542378
• Purification: Protein A purified
• Nucleotide Accession #: NM_080647
• Tested Species Reactivity: Human
• Gene Symbol: TBX1
• Predicted Species Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human kidney
  
  Human kidney
• Image 2: Human Jurkat
  
  WB Suggested Anti-TBX1 Antibody Titration: 5.0ug/ml
  Positive Control: Jurkat cell lysate
• Image 3:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.