| Product Number |
ARP33010_P050 |
| Product Page |
www.avivasysbio.com/ttrap-antibody-middle-region-arp33010-p050.html |
| Name |
TTRAP Antibody - middle region (ARP33010_P050) |
| Protein Size (# AA) |
362 amino acids |
| Molecular Weight |
41kDa |
| NCBI Gene Id |
51567 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Tyrosyl-DNA phosphodiesterase 2 |
| Description |
|
| Alias Symbols |
EAP2, AD022, EAPII, TTRAP, hTDP2, dJ30M3.3 |
| Peptide Sequence |
Synthetic peptide located within the following region: IPPYYSYLKKRSSNYEIITGHEEGYFTAIMLKKSRVKLKSQEIIPFPSTK |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Pei,H., et al., (2003) Oncogene 22 (18), 2699-2709 |
| Description of Target |
The TTRAP gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. |
| Protein Interactions |
UBC; SRPK2; SUMO1; SUMO2; SUMO3; TAP1; SKIL; ATXN1; PSEN1; SMAD3; EPS8; DLX3; ATP5G2; C2orf88; JAM2; SNX13; AKAP9; RAB11A; PARK7; MAPK6; UBE2I; TRAF5; TRAF6; TRAF3; TRAF2; FLI1; TNFRSF1B; ETS2; ETS1; TNFRSF8; CD40; HTNVsSgp1; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-TDP2 (ARP33010_P050) antibody |
| Blocking Peptide |
For anti-TDP2 (ARP33010_P050) antibody is Catalog # AAP33010 (Previous Catalog # AAPP04039) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human TTRAP |
| Uniprot ID |
O95551 |
| Protein Name |
Tyrosyl-DNA phosphodiesterase 2 |
| Publications |
Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res. (2016). 27789688
Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res. 45, 1159-1176 (2017). 28180300
Xu, G.-L. et al. TTRAP is a novel PML nuclear bodies-associated protein. Biochem. Biophys. Res. Commun. 375, 395-8 (2008). 18706885 |
| Sample Type Confirmation |
TDP2 is supported by BioGPS gene expression data to be expressed in HepG2 |
| Protein Accession # |
NP_057698 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_016614 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
TDP2 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 100% |
| Image 1 | r-tdp2
 | Sample: 1. DT40 (5x105cells)
2. DT40 + rec. hTDP2 (5x105cells)
3. soluble HIS-hTDP2 (5x105cells)
4. pellet HIS-hTDP2 (5x105cells)
Primary dilution:1:1000
Secondary Antibody: dakocytomation Goat anti-rabbit
Secondary dilution: 1:5000
Image Submitted by: Keith Caldecott
University of Sussex |
|
| Image 2 | Human HepG2
 | WB Suggested Anti-TTRAP Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysateTDP2 is supported by BioGPS gene expression data to be expressed in HepG2 |
|
| Image 3 | Human kidney
 | Human kidney |
|
