PPARG Antibody - N-terminal region (ARP32880_T100)

Data Sheet
 
Product Number ARP32880_T100
Product Page www.avivasysbio.com/pparg-antibody-n-terminal-region-arp32880-t100.html
Name PPARG Antibody - N-terminal region (ARP32880_T100)
Protein Size (# AA) 505 amino acids
Molecular Weight 58 kDa
NCBI Gene Id 5468
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Peroxisome proliferator-activated receptor gamma
Alias Symbols GLM1, CIMT1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma
Peptide Sequence Synthetic peptide located within the following region: SHSFDIKPFTTVDFSSISTPHYEDIPFTRTDPVVADYKYDLKLQEYQSAI
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Wang,Y., et al., (2006) Fertil. Steril. 85 (5), 1536-1540
Description of Target PPARG is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer.
Protein Interactions HDAC3; NCOA1; NCOA3; SUMO1; UBC; SUMO2; RXRA; MED1; HMGA1; EP300; NCOA2; CDC34; PRKAA2; PRKAA1; PPARGC1A; ASH2L; RBBP5; NSD1; NCOA6; PAXIP1; MUC1; NR3C1; SMAD2; CREBBP; ALOX15B; ZFYVE9; USP7; NCOR1; CDK5; SMARCD3; SMARCA4; NEDD4; BCAS2; MIF4GD; MED14; JAZ
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Enhanced Validation
Relative Expression (Western Blot) Avivasheild
Datasheets/Manuals Printable datasheet for anti-PPARG (ARP32880_T100) antibody
Blocking Peptide For anti-PPARG (ARP32880_T100) antibody is Catalog # AAP32880 (Previous Catalog # AAPP03904)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PPARG
Uniprot ID P37231
Protein Name Peroxisome proliferator-activated receptor gamma
Protein Accession # NP_056953
Purification Protein A purified
Nucleotide Accession # NM_015869
Tested Species Reactivity Human, Mouse
Gene Symbol PPARG
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 100%; Goat: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 93%
Image 1
Mouse cornea and eyelid
Mouse cornea and eyelid
Primary Antibody Dilution:
1:100
Secondary Antibody:
Goat anti-rabbit-AlexaFluor-546
Secondary Antibody Dilution:
1:1000
Color/Signal Descriptions:
PPARG: Green DAPI: Red
Gene Name:
Ppar Gamma
Submitted by:
Anonymous
Image 2

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Note the peptide sequence is 100% identical to the PPARG sequence with one mismatch, and a 43 kDa isoform also contains this peptide sequence.
 

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