| Product Number |
ARP32801_T100 |
| Product Page |
www.avivasysbio.com/pck1-antibody-middle-region-arp32801-t100.html |
| Name |
PCK1 Antibody - middle region (ARP32801_T100) |
| Protein Size (# AA) |
622 amino acids |
| Molecular Weight |
69kDa |
| NCBI Gene Id |
5105 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Phosphoenolpyruvate carboxykinase 1 (soluble) |
| Alias Symbols |
PCKDC, PEPCK1, PEPCKC, PEPCK-C |
| Peptide Sequence |
Synthetic peptide located within the following region: NGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAS |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Hajarnis,S., et al., (2005) J Biol Chem. 280(31), 28272-80 |
| Description of Target |
PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. |
| Protein Interactions |
UBC; FBXO25; C1R; APP; CUL5; BAG6; TCP1; TCEB1; SKP1; RPS15A; RPS6; RPS3A; RPS3; RPLP0; RPL4; PSMD12; PSMD2; PSMC5; PSMC4; PSMC2; PSMC1; PSMB7; PSMB4; PSMB1; PSMA5; PSMA2; PCK1; LDHA; HSPD1; HSPA1L; DNAJA1; HLA-C; HLA-B; GNAS; GNAI1; GNA12; FASN; EP300; T |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PCK1 (ARP32801_T100) antibody |
| Blocking Peptide |
For anti-PCK1 (ARP32801_T100) antibody is Catalog # AAP32801 |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human PCK1 |
| Uniprot ID |
P35558 |
| Protein Name |
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] |
| Protein Accession # |
NP_002582 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_002591 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
PCK1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 93%; Rabbit: 86%; Rat: 100% |
| Image 1 | Human kidney
 | Human kidney |
|
| Image 2 | Human Adult Placenta
 | Host: Rabbit
Target Name: PCK1
Sample Type: Human Adult Placenta
Antibody Dilution: 1.0ug/ml |
|
| Image 3 | Human Fetal Muscle
 | Host: Rabbit
Target Name: PCK1
Sample Type: Human Fetal Muscle
Antibody Dilution: 1.0ug/ml |
|
| Image 4 | Human Fetal Liver
 | Host: Rabbit
Target Name: PCK1
Sample Type: Human Fetal Liver
Antibody Dilution: 1.0ug/ml |
|
| Image 5 | Human Fetal Lung
 | Host: Rabbit
Target Name: PCK1
Sample Type: Human Fetal Lung
Antibody Dilution: 1.0ug/ml |
|
| Image 6 | Human Fetal Heart
 | Host: Rabbit
Target Name: PCK1
Sample Type: Human Fetal Heart
Antibody Dilution: 1.0ug/ml |
|
| Image 7 | Human MCF7
 | Host: Rabbit
Target Name: PCK1
Sample Type: MCF7
Antibody Dilution: 1.0ug/ml |
|
| Image 8 | Human MCF7 Whole Cell
 | Host: Rabbit
Target Name: PCK1
Sample Tissue: Human MCF7 Whole Cell
Antibody Dilution: 1ug/ml |
|
| Image 9 | Human 293T Whole Cell
 | Host: Rabbit
Target Name: PCK1
Sample Tissue: Human 293T Whole Cell
Antibody Dilution: 3ug/ml |
|
| Image 10 | Human Fetal Brain
 | WB Suggested Anti-PCK1 Antibody Titration: 1.0ug/ml
Positive Control: Fetal Brain Lysate |
|
| Image 11 | Human MCF7 Whole Cell
 | Host: Rabbit
Target Name: PCK1
Sample Tissue: Human MCF7 Whole Cell
Antibody Dilution: 1ug/ml |
|
