Product Number |
ARP32736_P050 |
Product Page |
www.avivasysbio.com/pax3-antibody-c-terminal-region-arp32736-p050.html |
Name |
PAX3 Antibody - C-terminal region (ARP32736_P050) |
Protein Size (# AA) |
479 amino acids |
Molecular Weight |
53kDa |
NCBI Gene Id |
5077 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Paired box 3 |
Alias Symbols |
WS1, WS3, CDHS, HUP2 |
Peptide Sequence |
Synthetic peptide located within the following region: GGVPHQPQTDYALSPLTGGLEPTTTVSASCSQRLDHMKSLDSLPTSQSYC |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Blake,J.A. (2005) Dev. Growth Differ. 47 (9), 627-635 |
Description of Target |
PAX3 is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma.This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. |
Protein Interactions |
PCTP; UBC; TAF1; POU3F2; HDAC1; TRIM28; HDAC10; SOX8; WWTR1; PAX3; DAXX; CIB1; SOX10; MEOX2; MEOX1; MITF; MSX1; Rad23b; PSMD4; TBP; IPO13; CNR1; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-PAX3 (ARP32736_P050) antibody |
Blocking Peptide |
For anti-PAX3 (ARP32736_P050) antibody is Catalog # AAP32736 (Previous Catalog # AAPP03750) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human PAX3 |
Uniprot ID |
P23760 |
Protein Name |
Paired box protein Pax-3 |
Protein Accession # |
NP_852122 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_181457 |
Tested Species Reactivity |
Human, Mouse |
Gene Symbol |
PAX3 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79% |
Image 1 | Human Lung
| WB Suggested Anti-PAX3 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positive Control: Human Lung |
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Image 2 | Mouse B16F10
| Sample Type: Mouse B16F10Primary Antibody Dilution: 1:200Secondary Antibody: Goat anti-rabbit-FITCSecondary Antibody Dilution: 1:800Color/Signal Descriptions: Green: FITC Blue: DAPIGene Name: PAX3Submitted by: Tsu Fang Wu, Institute of Molecular Biology, National Chung Hsing University |
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Image 3 | Human HEK293, A375, A2058, Mouse, B16F10
| WB Suggested Anti-PAX3 Antibody Positive Control: Lane 1: Flag-PAX3(overexpression, human), HEK293, 50?g. Lane 2: Mouse, B16F10, 50?g. Lane 3: Human, A375, 50?g. Lane 4: Human, A2058, 50?g Primary Antibody Dilution : 1:5000 Secondary Antibody : Goat anti-rabbit AP Secondry Antibody Dilution : 1:5000 Submitted by: Tsu Fang Wu, Institute of Molecular Biology, National Chung Hsing University |
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