Product Number |
ARP32615_T100 |
Product Page |
www.avivasysbio.com/hcls1-antibody-n-terminal-region-arp32615-t100.html |
Name |
HCLS1 Antibody - N-terminal region (ARP32615_T100) |
Protein Size (# AA) |
486 amino acids |
Molecular Weight |
54kDa |
NCBI Gene Id |
3059 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Hematopoietic cell-specific Lyn substrate 1 |
Alias Symbols |
HS1, p75, CTTNL, lckBP1 |
Peptide Sequence |
Synthetic peptide located within the following region: FGVERDRMDKSAVGHEYVAEVEKHSSQTDAAKGFGGKYGVERDRADKSAV |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Ruzzene,M., et al., (2002) Biochem. J. 364 (Pt 1), 41-47 |
Description of Target |
HS1 which is hematopoietic lineage cell-specific protein 1, is a substrate of protein tyrosine kinases in lymphocytes, it binds to F-actin, and promotes Arp2/3 complex-mediated actin polymerization. However, the mechanism for the interaction between HS1 and F-actin has not yet been fully characterized. HS1 contains 3.5 tandem repeats, a coiled-coil region, and an SH3 domain at the C terminus. Unlike cortactin, which is closely related to HS1 and requires absolutely the repeat domain for F-actin binding, an HS1 mutant with deletion of the repeat domain maintains a significant F-actin binding activity. Deletion of the coiled-coil region abolished the ability of HS1 to bind to actin filaments and to activate the Arp2/3 complex for actin nucleation and actin branching. |
Protein Interactions |
SH2D4A; UBC; Mbp; Dlg4; NOTCH1; QKI; TERF1; TRIM29; SSBP3; BLZF1; IKBKG; LZTR1; ZBTB25; HS1BP3; HAX1; ACTR2; MAP4K1; CASP3; SYK; WAS; LYN; FGR; CSNK2A1; CD79A; ACTA1; GRB2; ACTR3; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-HCLS1 (ARP32615_T100) antibody |
Blocking Peptide |
For anti-HCLS1 (ARP32615_T100) antibody is Catalog # AAP32615 (Previous Catalog # AAPP03624) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human HCLS1 |
Uniprot ID |
P14317 |
Protein Name |
Hematopoietic lineage cell-specific protein |
Sample Type Confirmation |
HCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat |
Protein Accession # |
NP_005326 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_005335 |
Tested Species Reactivity |
Human |
Gene Symbol |
HCLS1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 92%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93% |
Image 1 | Human Jurkat
| WB Suggested Anti-HCLS1 Antibody Titration: 1.25ug/ml Positive Control: Jurkat cell lysateHCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat |
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