| Product Number |
ARP32589_P050 |
| Product Page |
www.avivasysbio.com/chek1-antibody-middle-region-arp32589-p050.html |
| Name |
CHEK1 Antibody - middle region (ARP32589_P050) |
| Protein Size (# AA) |
476 amino acids |
| Molecular Weight |
54kDa |
| NCBI Gene Id |
1111 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Checkpoint kinase 1 |
| Alias Symbols |
CHK1 |
| Peptide Sequence |
Synthetic peptide located within the following region: WSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLAL |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Kramer,A., et al., (2004) Nat. Cell Biol. 6 (9), 884-891 |
| Description of Target |
CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint. |
| Protein Interactions |
RELA; CUL4A; UBC; SETMAR; DDB1; TNRC6B; ATR; HECW2; CLSPN; DTL; BTG3; TOPBP1; MAPKAPK2; HSP90AA1; CDC25C; RAD51; PIGM; CUL1; VCP; CDC25A; ATM; APP; MED15; NEK2; TLK1; RB1; BRCA1; FEM1B; XIAP; COPS5; MEPE; LATS2; TP53; Chek1; MEN1; USP7; DNMT1; FANCE; PCNA |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-CHEK1 (ARP32589_P050) antibody |
| Blocking Peptide |
For anti-CHEK1 (ARP32589_P050) antibody is Catalog # AAP32589 (Previous Catalog # AAPP03592) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human CHEK1 |
| Uniprot ID |
O14757 |
| Protein Name |
Serine/threonine-protein kinase Chk1 |
| Sample Type Confirmation |
CHEK1 is supported by BioGPS gene expression data to be expressed in HepG2 |
| Protein Accession # |
NP_001265 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001274 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
CHEK1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79% |
| Image 1 | Human HepG2
 | WB Suggested Anti-CHEK1 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysateCHEK1 is supported by BioGPS gene expression data to be expressed in HepG2 |
|
| Image 2 | Human kidney
 | Human kidney |
|
